自然流产与正常妊娠滋养细胞凋亡活性的差异。

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Accounts of Chemical Research Pub Date : 2022-01-01 Epub Date: 2022-01-17 DOI:10.5603/FHC.a2022.0003
Theodora-Eleftheria Deftereou, Avgi Tsolou, Anastasios Liberis, Kyriaki Georgiadi, Triantafyllos Alexiadis, Olga Pagonopoulou, Christina-Angelika Alexiadi, Maria Simopoulou, Grigorios Tripsianis, Maria Lambropoulou
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引用次数: 0

摘要

细胞凋亡是正常滋养层发育乃至整个妊娠过程的关键过程。然而,在自然流产的滋养细胞分化过程中,对细胞凋亡的研究很少。因此,本研究旨在探讨滋养细胞凋亡频率与自然流产发生率之间的关系。材料和方法:采用免疫组化方法对72例滋养细胞标本进行免疫组化检测。72例中有42例来自妊娠早期自然流产,其余30例来自妊娠同期的选择性终止妊娠。TUNEL法和M30标记法免疫组化评价细胞凋亡。结果:自然流产和择期终止妊娠组织的比较研究表明,与正常妊娠相比,自然流产组织中两种凋亡标志物的表达均增加。此外,统计分析表明,产妇年龄和妊娠与自然流产发生率增加有关。此外,M30和TUNEL染色与自然流产患者的产妇年龄和原产性显著相关。结论:我们的数据证明,妊娠前三个月细胞凋亡活性升高显然与自然流产有关。此外,两种成熟的凋亡标志物在评估流产后组织方面显示出很高的统计学意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Differential apoptotic activity in trophoblast of spontaneous abortions and normal pregnancies.

Introduction: Apoptosis is a key process during normal trophoblastic development and, consequently, the whole gestation. However, in trophoblastic differentiation in spontaneous abortions apoptosis has been hardly investigated. Therefore, the aim of the study was to investigate the correlation between apoptotic frequency in trophoblast and spontaneous abortion incidences.

Material and methods: A total of 72 trophoblastic tissue samples were immunohistochemically examined. 42 of 72 derived from first-trimester spontaneous abortions and the remaining 30 from elective terminations during the same trimester of pregnancy. TUNEL assay and M30 marker were used for apoptosis evaluation by immunohistochemistry.

Results: Comparative study of tissues from spontaneous abortions and elective pregnancy terminations demonstrated increased expression of both apoptotic markers in tissues derived from spontaneous abortions compared to normal pregnancies. In addition, statistical analysis correlated maternal age and gravidity with increased spontaneous abortion incidences. Moreover, both M30 and TUNEL staining were significantly correlated with maternal age and primigravidity in spontaneous abortion cases.

Conclusions: Our data proved that elevated apoptotic activity during the first pregnancy trimester is clearly involved in spontaneous abortions. Moreover, two well-established apoptotic markers revealed high statistical significance in the evaluation of post-abortive tissues.

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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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