{"title":"从地下盐晶体中分离出一种新的头尾盐病毒并感染盐丛。","authors":"Shaoxing Chen, Yongpei Dai, Jingwen Liu, Shimin Zhang, Feilong Chen, Fanjie Jin, Peiyao Ruan, Lu Li, Xiangdong Chen","doi":"10.1155/2021/8271899","DOIUrl":null,"url":null,"abstract":"<p><p>A novel halovirus, VOLN27B, was isolated from a drill core sample taken at a depth of approximately 430 m, from a layer formed during the Cretaceous period (Anhui, China). VOLN27B infects the halophilic archaeon <i>Halorubrum</i> sp. LN27 and has a head-tailed morphotype with a contractile tail, typical of myoviruses. The average head diameter is 64 ± 2.0 nm, and uncontracted tails are 15 ± 1.0 × 65 ± 2.0 nm. The latent period is about 10 h. The maturing time of VOLN27B in cells of <i>Halorubrum</i> sp. LN27 was nearly 8 h. The adsorption time of VOLN27B on cells of <i>Halorubrum</i> sp. LN27 was less than 1 min. Virus particles are unstable at pH values less than 5 or when the NaCl concentration is below 12% (<i>w</i>/<i>v</i>). VOLN27B and <i>Halorubrum</i> sp. LN27 were recovered from the same hypersaline environment and provide a new virus-host system in haloarchaea.</p>","PeriodicalId":49105,"journal":{"name":"Archaea-An International Microbiological Journal","volume":"2021 ","pages":"8271899"},"PeriodicalIF":2.3000,"publicationDate":"2021-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8727067/pdf/","citationCount":"3","resultStr":"{\"title\":\"VOLN27B: A New Head-Tailed Halovirus Isolated from an Underground Salt Crystal and Infecting <i>Halorubrum</i>.\",\"authors\":\"Shaoxing Chen, Yongpei Dai, Jingwen Liu, Shimin Zhang, Feilong Chen, Fanjie Jin, Peiyao Ruan, Lu Li, Xiangdong Chen\",\"doi\":\"10.1155/2021/8271899\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>A novel halovirus, VOLN27B, was isolated from a drill core sample taken at a depth of approximately 430 m, from a layer formed during the Cretaceous period (Anhui, China). VOLN27B infects the halophilic archaeon <i>Halorubrum</i> sp. LN27 and has a head-tailed morphotype with a contractile tail, typical of myoviruses. The average head diameter is 64 ± 2.0 nm, and uncontracted tails are 15 ± 1.0 × 65 ± 2.0 nm. The latent period is about 10 h. The maturing time of VOLN27B in cells of <i>Halorubrum</i> sp. LN27 was nearly 8 h. The adsorption time of VOLN27B on cells of <i>Halorubrum</i> sp. LN27 was less than 1 min. Virus particles are unstable at pH values less than 5 or when the NaCl concentration is below 12% (<i>w</i>/<i>v</i>). VOLN27B and <i>Halorubrum</i> sp. LN27 were recovered from the same hypersaline environment and provide a new virus-host system in haloarchaea.</p>\",\"PeriodicalId\":49105,\"journal\":{\"name\":\"Archaea-An International Microbiological Journal\",\"volume\":\"2021 \",\"pages\":\"8271899\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2021-12-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8727067/pdf/\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Archaea-An International Microbiological Journal\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1155/2021/8271899\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2021/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q3\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Archaea-An International Microbiological Journal","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1155/2021/8271899","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2021/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
VOLN27B: A New Head-Tailed Halovirus Isolated from an Underground Salt Crystal and Infecting Halorubrum.
A novel halovirus, VOLN27B, was isolated from a drill core sample taken at a depth of approximately 430 m, from a layer formed during the Cretaceous period (Anhui, China). VOLN27B infects the halophilic archaeon Halorubrum sp. LN27 and has a head-tailed morphotype with a contractile tail, typical of myoviruses. The average head diameter is 64 ± 2.0 nm, and uncontracted tails are 15 ± 1.0 × 65 ± 2.0 nm. The latent period is about 10 h. The maturing time of VOLN27B in cells of Halorubrum sp. LN27 was nearly 8 h. The adsorption time of VOLN27B on cells of Halorubrum sp. LN27 was less than 1 min. Virus particles are unstable at pH values less than 5 or when the NaCl concentration is below 12% (w/v). VOLN27B and Halorubrum sp. LN27 were recovered from the same hypersaline environment and provide a new virus-host system in haloarchaea.
期刊介绍：
Archaea is a peer-reviewed, Open Access journal that publishes original research articles as well as review articles dealing with all aspects of archaea, including environmental adaptation, enzymology, genetics and genomics, metabolism, molecular biology, molecular ecology, phylogeny, and ultrastructure. Bioinformatics studies and biotechnological implications of archaea will be considered. Published since 2002, Archaea provides a unique venue for exchanging information about these extraordinary prokaryotes.
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