基于适应症的机械间质细胞移植方案的不同解决方案。

Scars, burns & healing Pub Date : 2022-01-05 eCollection Date: 2022-01-01 DOI:10.1177/20595131211047830
H Eray Copcu
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引用次数: 3

摘要

背景:再生医学是近年来发展最快的整形外科分支。脂肪组织是体内最大和最重要的基质细胞来源之一。虽然机械隔离方法非常流行,并且有许多优点,但它们仍然没有公认的协议。目的:我们通过使用超锋利刀片和用不同溶液(生理盐水、林格和5%葡萄糖)稀释脂肪组织,开发了标准化的新方案,并开发了名为机械基质细胞转移(MEST)的新技术。方法和材料:为了获得所需的物理结构(液体、凝胶、固体)和所需的体积,我们定义了四种不同类型的IPs。在ip1和ip2中使用10或20 cc注射器用不同的溶液预稀释脂肪组织,而在ip3和ip4中直接使用浓缩脂肪组织。结果:在MEST中,使用不同的IPs从100 mL浓缩脂肪中获得基质细胞,平均存活率为92%,细胞计数为26.80 ~ 91.90 × 106。通过使用四种不同的诱导能,可以获得所需形态和数量的基质细胞。结论:用锋利的刀片切割脂肪分离基质细胞,可以防止脂肪组织和基质细胞的死亡,具有较高的细胞存活率和细胞计数。用不同的溶液预稀释:在加盐之前,用所需的溶液(生理盐水,林格或5%葡萄糖)稀释浓缩的脂肪组织,可以提供更多的基质细胞。摘要:从脂肪组织中获得再生基质细胞有两种方法:酶法和机械法。机械方法有许多优点。尽管从脂肪组织中机械提取基质细胞非常流行,并且已经描述了许多技术,但仍然没有公认的方案,最终产品的定义,并且对基质细胞的状态没有达成共识。在这项研究中,通过使用超锋利的刀片系统机械地获得基质细胞,而不会将脂肪组织暴露在钝性创伤中。因此,可以获得更多的细胞数量和更高的活力。为了获得所需的数量和状态(固体、半固体、液体)最终产物,首次定义了“基于适应症”的方案。用所需的溶液(生理盐水,林格或5%葡萄糖)稀释浓缩脂肪组织,然后再进行加脂处理,这样可以提供更多的基质细胞。这将为临床医生获得和应用基质细胞溶液在不同解剖区域的不同适应症提供机会。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Indication-based protocols with different solutions for mechanical stromal-cell transfer.

Indication-based protocols with different solutions for mechanical stromal-cell transfer.

Indication-based protocols with different solutions for mechanical stromal-cell transfer.

Indication-based protocols with different solutions for mechanical stromal-cell transfer.

Background: Regenerative medicine is the fastest developing branch of plastic surgery in recent times. Adipose tissue is one of the largest and most important sources in the body for stromal cells. Although mechanical isolation methods are both very popular and have many advantages, they still have no accepted protocols.

Objective: We developed new protocols called indication-based protocols (IPs) for standardization and new techniques called mechanical stromal-cell transfer (MEST) by using ultra-sharp blades and dilution of adipose tissue with different solutions (saline, Ringer and 5% Dextrose)Methods & material: In order to obtain the desired physical structure (liquid, gel, solid) and the desired volume, four different types of IPs have been defined. Adipose tissue was prediluted with different solutions using 10 or 20 cc injectors in IPs 1 and 2, while condensed adipose tissue was used directly in IPs 3 and 4.

Results: In MEST, stromal cells were obtained from 100 mL of condensed fat using different IPs with 92% mean viability and cell counts of 26.80-91.90 × 106. Stromal cells can be obtained in the desired form and number of cells by using four different IPs.

Conclusion: Isolation of stromal cells by cutting fat with sharp blades will prevent the death of fat tissue and stromal cells and will allow high viability and cell count with our new technique. Predilution with different solutions: Diluting the condensed adipose tissue with the desired solutions (saline, Ringer or 5% Dextrose) before the adinizing process will provide even more stromal cells.

Lay summary: Obtaining regenerative stromal cells from adipose tissue can be done by two methods: Enzymatic and mechanical. Mechanical methods have many advantages. Although mechanical stromal cell extraction from adipose tissue is very popular and many techniques have been described, there are still no accepted protocols, definition for the end product, and no consensus on the status of the stromal cells. In this study, stromal cells were obtained mechanically by using ultra-sharp blade systems, without exposing adipose tissue to blunt trauma. Thus, a higher number of cells and higher viability could be obtained. An "Indication based" protocol has been defined for the first time in order to obtain the desired number and status (solid, semi-solid, liquid)end product. Diluting the condensed adipose tissue with the desired solutions (saline, Ringer or 5% Dextrose) before the adinizing process will provide even more stromal cells. This will provide an opportunity for clinicians to obtain and apply a stromal cell solution for different indications in different anatomical regions.

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