同位素标记在细胞器蛋白定位中的应用现状及在药物发现研究中的潜在应用

Q1 Pharmacology, Toxicology and Pharmaceutics

Drug Discovery Today: Technologies Pub Date : 2021-12-01 DOI:10.1016/j.ddtec.2021.06.003

Mohamed A.W. Elzek , Josie A. Christopher , Lisa M. Breckels , Kathryn S. Lilley

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引用次数: 4

摘要

空间蛋白质组学为蛋白质功能和亚细胞定位之间的关系提供了重要的见解。通过同位素标记(LOPIT)及其变体定位细胞器蛋白是蛋白质组范围的技术，在规模上与基于显微镜或基于接近标记的技术不匹配，允许全面定位蛋白质亚细胞位置和细胞扰动下游的重新定位事件。LOPIT可以成为药物发现的一个强大而通用的工具，用于解锁疾病病理生理学，药物作用机制和脱靶毒性筛选的重要信息。本文讨论了LOPIT的技术概念及其在药物发现和开发研究中的潜在应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Localization of Organelle Proteins by Isotope Tagging: Current status and potential applications in drug discovery research

Spatial proteomics has provided important insights into the relationship between protein function and subcellular location. Localization of Organelle Proteins by Isotope Tagging (LOPIT) and its variants are proteome-wide techniques, not matched in scale by microscopy-based or proximity tagging-based techniques, allowing holistic mapping of protein subcellular location and re-localization events downstream of cellular perturbations. LOPIT can be a powerful and versatile tool in drug discovery for unlocking important information on disease pathophysiology, drug mechanism of action, and off-target toxicity screenings. Here, we discuss technical concepts of LOPIT with its potential applications in drug discovery and development research.

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来源期刊

Drug Discovery Today: Technologies Pharmacology, Toxicology and Pharmaceutics-Drug Discovery

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期刊介绍： Discovery Today: Technologies compares different technological tools and techniques used from the discovery of new drug targets through to the launch of new medicines.