石榴皮乙醇提取物对dss诱导小鼠Caspase-3表达的影响。

IF 2.6 Q3 IMMUNOLOGY
International Journal of Inflammation Pub Date : 2021-12-02 eCollection Date: 2021-01-01 DOI:10.1155/2021/4919410
Kusmardi Kusmardi, Lyanna Azzahra Baihaqi, Ari Estuningtyas, Nurhuda Sahar, Hadi Sunaryo, Aryo Tedjo
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引用次数: 5

摘要

背景:结直肠癌(Colorectal cancer, CRC)是一种起源于结肠腺上皮细胞的恶性肿瘤。炎症性肠病(IBD)患者更容易发展为结直肠癌。肿瘤增殖的特点是丧失对凋亡的抑制,这涉及到caspase-3的激活。研究了石榴皮提取物对2%葡聚糖硫酸钠(DSS)诱导小鼠隐窝细胞caspase-3表达的影响。方法:实验研究分为6组。所有治疗均在42天内完成。除正常组只给予水外,其余各组均通过饮水诱导DSS。给予的治疗包括两种剂量的石榴提取物(240毫克和480毫克/公斤体重/天),阿司匹林和鞣花酸。将标本固定染色,进行caspase-3表达的免疫组化评分,并进行统计学分析。结果:各治疗组h评分分别为213.23±8.32 (DSS组)、243.81±18.69(正常组)、226.10±12.38(石榴皮提取物240 mg/kg/d)、238.84±15.81(石榴皮提取物480 mg/kg/d)、227.47±12.15(阿司匹林)、224.01±18.39(褪黑酸)。DSS组、正常组和剂量2组(石榴皮提取物480 mg/kg/day)的单因素方差分析和事后分析均有统计学差异。结论:石榴醇提物具有诱导细胞凋亡的作用,其表现为caspase-3表达的增加。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Ethanol Extract of Pomegranate (<i>Punica granatum</i>) Peel in Increasing the Expression of Caspase-3 in DSS-Induced Mice.

Ethanol Extract of Pomegranate (<i>Punica granatum</i>) Peel in Increasing the Expression of Caspase-3 in DSS-Induced Mice.

Ethanol Extract of Pomegranate (<i>Punica granatum</i>) Peel in Increasing the Expression of Caspase-3 in DSS-Induced Mice.

Ethanol Extract of Pomegranate (Punica granatum) Peel in Increasing the Expression of Caspase-3 in DSS-Induced Mice.

Background: Colorectal cancer (CRC) is a malignancy derived from the glandular epithelial cells in the colon. Patients with inflammatory bowel disease (IBD) are more likely to develop CRC. Cancer proliferation is characterized by the loss of inhibition of apoptosis, which involves caspase-3 activation. This study examined the effects of the pomegranate peel extract on the expression of caspase-3 in mice crypt cells induced by dextran sodium sulfate (DSS) 2%.

Methods: The experimental study was done in six groups. All treatments were done in 42 days. The groups were all induced by DSS through water drinking, except for the normal group, which was only given water. The treatments given included the pomegranate extract in two doses (240 mg and 480 mg/kg bw/day), aspirin, and ellagic acid. The specimens were then fixated and stained for the immunohistochemistry scoring for the expression of caspase-3, which was then analyzed statistically.

Results: The H-scores of each treatment group were 213.23 ± 8.32 (DSS group), 243.81 ± 18.69 (normal group), 226.10 ± 12.38 (pomegranate peel extract of 240 mg/kg/d), 238.84 ± 15.81 (pomegranate peel extract of 480 mg/kg/d), 227.47 ± 12.15 (aspirin), and 224.01 ± 18.39 (ellagic acid). Statistical differences were found in one-way analysis of variance (ANOVA) and post hoc analysis among the DSS group, normal group, and dose 2 group (pomegranate peel extract of 480 mg/kg/day).

Conclusions: The ethanol extract of pomegranate was able to induce apoptosis, which was demonstrated by the increase of caspase-3 expression.

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来源期刊
CiteScore
3.80
自引率
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发文量
16
审稿时长
16 weeks
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