同时评估有氧运动男性在有或无碳水化合物溶液的情况下进行长时间运动后唾液和尿液中的α-淀粉酶活性。

Pub Date : 2021-08-28
Nobuo Yasuda, Kohei Yamamoto, Naoki Iwashita
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引用次数: 0

摘要

研究目的本研究的目的是确定在摄入碳水化合物或水安慰剂的情况下进行 2 小时适度延长的运动对训练有素的男性唾液和尿α-淀粉酶同工酶活性的影响:十一名有氧健身男子参加了这项研究。在两个不同的场合,参与者以 60% 的峰值摄氧量进行了 2 小时的恒定功率输出自行车运动。研究设计包括随机顺序、安慰剂对照和交叉分配。在 2 小时的耐力运动中,参与者每二十分钟饮用 6.2% 的碳水化合物溶液或安慰剂水(每公斤体重 2 毫升)。在运动前后 10 分钟内,采用被动流口水法采集未受刺激的全唾液样本,用于定量检测唾液中的α-淀粉酶、免疫球蛋白 A(IgA)和总蛋白。在运动前(-2-0 小时)、运动后立即(0-2 小时)和运动后 24-26 小时三个时间点采集两小时尿液样本,分析α-淀粉酶同工酶活性(胰腺和唾液来源类型):结果:中度持久运动后,唾液和尿液中的α-淀粉酶活性与唾液总蛋白浓度相关性显著增加,但无论是否摄入外源性碳水化合物,唾液IgA浓度均无统计学意义:这些研究结果表明,无论是否摄入外源性碳水化合物,2 小时的适度运动似乎都会导致唾液和尿液中的α-淀粉酶活性增强,这表明粘膜免疫防御能力增强。
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Concurrent evaluation of salivary and urinary α-amylase activity following prolonged exercise with or without carbohydrate solution in aerobically active men.

Objective: The purpose of this study was to determine the effects of 2-h moderately prolonged exercise with carbohydrate intake or water placebo on salivary and urinary α-amylase isoenzyme activity in trained men.

Materials and methods: Eleven aerobically fit men participated in this study. On two different occasions, participants performed 2-h cycling corresponding to a constant power output at 60% peak oxygen uptake. The study design involved a random order, placebo-controlled and cross-over assignment. Participants consumed either 6.2% carbohydrate solution or water placebo every twenty minutes thereafter (2 ml/kg body mass) over 2-h endurance exercise. Unstimulated whole salivary samples were collected using the passive drooling method at the 10-min period before and after exercise for the quantification of salivary α-amylase, immunoglobulin A (IgA) and total protein. Two-hour urinary samples were obtained at three time points before (-2-0h), immediately (0-2 h) after and 24-26 h after exercise for the analysis of α-amylase isoenzyme activity (pancreas- and saliva-derived types).

Results: The activity of α-amylase in saliva and urine was significantly increased in connect with salivary total protein concentration immediately after moderately long-lasting exercise, but salivary IgA concentration was not statistically significant with or without exogenous carbohydrate intake.

Conclusion: These findings suggest that 2-h moderate exercise appears to lead to the enhanced α-amylase activity in saliva and urine regardless of exogenous carbohydrate availability, demonstrating enhanced mucosal immune defense.

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