{"title":"全氟辛酸对人肝癌细胞毒性的测定。","authors":"Mahmoud Abudayyak, Ezgi Öztaş, Gül Özhan","doi":"10.5696/2156-9614-11.31.210909","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Perfluorooctanoic acid (PFOA) is used in different industrial and commercial products. Research shows the presence of PFOA in home dusts, tap and surface water, and in biological samples. The International Agency for Research on Cancer (IARC) has classified PFOA as a possible carcinogen for humans. The liver is thought to be a target organ of PFOA accumulation and toxicity.</p><p><strong>Objective: </strong>Some studies have found toxic effects on the liver and related mechanisms; however, more studies are needed to better understand PFOA - induced hepatotoxicity.</p><p><strong>Methods: </strong>In the present study, a human hepatocarcinoma cell line was exposed to PFOA for 24 hours and cell viability, apoptosis, the oxidative system and immune response were evaluated.</p><p><strong>Results: </strong>While apoptosis was the main cell death pathway at low concentration (86.5%), the necrotic cell fraction increased with higher concentrations (46.7%). Significant changes in the reactive oxygen species (5.3-folds) glutathione (GSH) (1.7-folds) and catalase (CAT) (1.4-folds) levels were observed, as well as changes to interleukin-6 (≤1.8-fold) and interleukin-8 levels (35-40%).</p><p><strong>Conclusions: </strong>In light of the data, PFOA is potentially hepatotoxic through the investigated pathways. The results represent a background for future in vivo mechanistic studies.</p><p><strong>Competing interests: </strong>The authors declare no competing financial interests.</p>","PeriodicalId":52138,"journal":{"name":"Journal of Health and Pollution","volume":" ","pages":"210909"},"PeriodicalIF":2.4000,"publicationDate":"2021-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8383792/pdf/","citationCount":"4","resultStr":"{\"title\":\"Determination of Perflourooctanoic Acid Toxicity in a Human Hepatocarcinoma Cell Line.\",\"authors\":\"Mahmoud Abudayyak, Ezgi Öztaş, Gül Özhan\",\"doi\":\"10.5696/2156-9614-11.31.210909\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Perfluorooctanoic acid (PFOA) is used in different industrial and commercial products. Research shows the presence of PFOA in home dusts, tap and surface water, and in biological samples. The International Agency for Research on Cancer (IARC) has classified PFOA as a possible carcinogen for humans. The liver is thought to be a target organ of PFOA accumulation and toxicity.</p><p><strong>Objective: </strong>Some studies have found toxic effects on the liver and related mechanisms; however, more studies are needed to better understand PFOA - induced hepatotoxicity.</p><p><strong>Methods: </strong>In the present study, a human hepatocarcinoma cell line was exposed to PFOA for 24 hours and cell viability, apoptosis, the oxidative system and immune response were evaluated.</p><p><strong>Results: </strong>While apoptosis was the main cell death pathway at low concentration (86.5%), the necrotic cell fraction increased with higher concentrations (46.7%). Significant changes in the reactive oxygen species (5.3-folds) glutathione (GSH) (1.7-folds) and catalase (CAT) (1.4-folds) levels were observed, as well as changes to interleukin-6 (≤1.8-fold) and interleukin-8 levels (35-40%).</p><p><strong>Conclusions: </strong>In light of the data, PFOA is potentially hepatotoxic through the investigated pathways. The results represent a background for future in vivo mechanistic studies.</p><p><strong>Competing interests: </strong>The authors declare no competing financial interests.</p>\",\"PeriodicalId\":52138,\"journal\":{\"name\":\"Journal of Health and Pollution\",\"volume\":\" \",\"pages\":\"210909\"},\"PeriodicalIF\":2.4000,\"publicationDate\":\"2021-08-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8383792/pdf/\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Health and Pollution\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5696/2156-9614-11.31.210909\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2021/9/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q1\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Health and Pollution","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5696/2156-9614-11.31.210909","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2021/9/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"Medicine","Score":null,"Total":0}
Determination of Perflourooctanoic Acid Toxicity in a Human Hepatocarcinoma Cell Line.
Background: Perfluorooctanoic acid (PFOA) is used in different industrial and commercial products. Research shows the presence of PFOA in home dusts, tap and surface water, and in biological samples. The International Agency for Research on Cancer (IARC) has classified PFOA as a possible carcinogen for humans. The liver is thought to be a target organ of PFOA accumulation and toxicity.
Objective: Some studies have found toxic effects on the liver and related mechanisms; however, more studies are needed to better understand PFOA - induced hepatotoxicity.
Methods: In the present study, a human hepatocarcinoma cell line was exposed to PFOA for 24 hours and cell viability, apoptosis, the oxidative system and immune response were evaluated.
Results: While apoptosis was the main cell death pathway at low concentration (86.5%), the necrotic cell fraction increased with higher concentrations (46.7%). Significant changes in the reactive oxygen species (5.3-folds) glutathione (GSH) (1.7-folds) and catalase (CAT) (1.4-folds) levels were observed, as well as changes to interleukin-6 (≤1.8-fold) and interleukin-8 levels (35-40%).
Conclusions: In light of the data, PFOA is potentially hepatotoxic through the investigated pathways. The results represent a background for future in vivo mechanistic studies.
Competing interests: The authors declare no competing financial interests.
期刊介绍：
The Journal of Health and Pollution (JH&P) was initiated with funding from the European Union and World Bank and continues to be a Platinum Open Access Journal. There are no publication or viewing charges. That is, there are no charges to readers or authors. Upon peer-review and acceptance, all articles are made available online. The high-ranking editorial board is comprised of active members who participate in JH&P submissions and editorial policies. The Journal of Health and Pollution welcomes manuscripts based on original research as well as findings from re-interpretation and examination of existing data. JH&P focuses on point source pollution, related health impacts, environmental control and remediation technology. JH&P also has an interest in ambient and indoor pollution. Pollutants of particular interest include heavy metals, pesticides, radionuclides, dioxins, polychlorinated biphenyls (PCBs), polycyclic aromatic hydrocarbons (PAHs), volatile organic compounds (VOCs), air particulates (PM10 and PM2.5), and other severe and persistent toxins. JH&P emphasizes work relating directly to low and middle-income countries, however relevant work relating to high-income countries will be considered on a case-by-case basis.
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