Halida Thanveer Asana Marican, Lucas W H Sun, Hongyuan Shen
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A Simple Method to Establish Metaphase Chromosomes from Individual Zebrafish Embryos.
Cytogenetic approach based on metaphase chromosomes established from dividing cells enables direct microscopic visualization of individual chromosomes, a powerful technique to investigate aneuploidy, chromosome aberrations, and genomic instability. In this study, we describe a simple method based on direct blocking of metaphases in individual zebrafish embryo and dropping slides with temperature changes, water vapor, and acetic acid treatment to increase the metaphase diameters. We demonstrate that well-separated metaphases could be established from single zebrafish embryos using this method. Our method could be further adapted for the analyses of DNA damage, chromosome aberrations, and genomic instability using zebrafish and other teleost models.
期刊介绍:
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.