血清和胸腔液中一组mirna对恶性和良性胸腔积液鉴别诊断的评估。

IF 1.9
Li-Rong Zhu, Rong-Xia Yuan, Xian-Bin Xia, Yi Wang, Yu-Min Zhu, Ling Fi, Jian Li
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引用次数: 1

摘要

背景:恶性胸腔积液(MPE)和良性胸腔积液(BPE)的鉴别诊断仍然是一个临床挑战。目的:评价血清和胸膜液(PF) miRNA检测在MPE和BPE鉴别中的作用。方法:采用实时荧光定量PCR (quantitative real-time PCR, qRT-PCR)检测20例MPE和20例BPE患者中14个在肺癌中表达异常的候选mirna。在所有参与本研究的患者中,通过qRT-PCR进一步评估显著异常表达的mirna。构建受试者工作特征(ROC)曲线,计算ROC曲线下面积(AUC),评价mirna的诊断效能。结果:发现miR-21、miR-29c和miR-182在MPE患者血清和PF中显著异常表达。血清和PF中miR-21、miR-29c和miR-182联合应用的MPE与结核性胸膜炎、肺炎旁肺等感染相关PE的AUC分别为0.832和0.89,MPE与心力衰竭相关PE的AUC分别为0.866和0.919,均优于各mirna的AUC。结论:血清和PF中的miR-21、miR-29c和miR-182可能是诊断MPE的有用生物标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Assessment of a panel of miRNAs in serum and pleural fluid for the differential diagnosis of malignant and benign pleural effusion.

Background: Differential diagnosis between malignant pleural effusion (MPE) and benign pleural effusion (BPE) remains a clinical challenge.

Objective: The aim of the study is to assess the efficacy of the serum and pleural fluid (PF) miRNA panels in distinguishing MPE from BPE.

Methods: Fourteen candidate miRNAs which were shown aberrant expression in lung cancer based on previous studies were tested by quantitative real-time PCR (qRT-PCR) in 20 MPE patients and 20 BPE patients. Significantly aberrantly expressed miRNAs were further assessed by qRT-PCR in all patients enrolled in this study. A receiver operating characteristic (ROC) curve was constructed, and the area under the ROC curve (AUC) was calculated to evaluated the diagnostic performance of the miRNAs.

Results: miR-21, miR-29c and miR-182 were found to be significantly aberrantly expressed in the serum and PF of MPE patients. The AUCs for the combination of miR-21, miR-29c and miR-182 in serum and PF were 0.832 and 0.89 respectively in distinguishing MPE from infection-associated PE including tuberculous pleurisy and parapneumonia PE, and 0.866 and 0.919 respectively for differentiating MPE from heart failure-associated PE, which were superior to AUC of each individual miRNAs.

Conclusions: miR-21, miR-29c and miR-182 in serum and PF could be useful biomarkers for diagnosis of MPE.

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