{"title":"hns mRNA下调galU的表达,减弱伤寒沙门氏菌血清型的活动性","authors":"Xin Zhao , Fanfan Yang , Yurou Wang , Ying Zhang","doi":"10.1016/j.ijmm.2021.151525","DOIUrl":null,"url":null,"abstract":"<div><p>Recently, multiple bifunctional RNAs have been discovered, which can both be translated into proteins and play regulatory roles. <em>hns</em> encodes the global gene silencing factor H-NS, which is widespread in Gram-negative bacteria. This study reported that <em>hns</em> mRNA of <em>Salmonella enterica</em> serovar Typhi (<em>S</em>. Typhi) was a bifunctional RNA that could act as an antisense RNA downregulating the expression of <em>galU</em>, the coding gene of uridine triphosphate-glucose-1-phosphate uridylyltransferase, and attenuating bacterial motility. <em>galU</em>, which is located at the opposite strand of <em>hns</em>, was identified to have a long 3′-untranslated region that overlapped with <em>hns</em> and could be processed to produce short RNA fragments. The overexpression of <em>hns</em> mRNA inhibited the expression of <em>galU</em>. The deletion of <em>galU</em> attenuated the motility of <em>S.</em> Typhi, while the complementation of <em>galU</em> nearly restored the phenotype. Overexpressing <em>hns</em> mRNA in the wild-type strain of <em>S.</em> Typhi inhibited the motility and the expression of flagellar genes, while overexpressing <em>hns</em> mRNA in the <em>galU</em>-deletion mutant did not influence bacterial motility. In conclusion, <em>hns</em> mRNA has been identified to be a new bifunctional RNA that attenuates the motility of <em>S.</em> Typhi by downregulating the expression of <em>galU</em>.</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"311 6","pages":"Article 151525"},"PeriodicalIF":4.5000,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ijmm.2021.151525","citationCount":"2","resultStr":"{\"title\":\"hns mRNA downregulates the expression of galU and attenuates the motility of Salmonella enterica serovar Typhi\",\"authors\":\"Xin Zhao , Fanfan Yang , Yurou Wang , Ying Zhang\",\"doi\":\"10.1016/j.ijmm.2021.151525\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Recently, multiple bifunctional RNAs have been discovered, which can both be translated into proteins and play regulatory roles. <em>hns</em> encodes the global gene silencing factor H-NS, which is widespread in Gram-negative bacteria. This study reported that <em>hns</em> mRNA of <em>Salmonella enterica</em> serovar Typhi (<em>S</em>. Typhi) was a bifunctional RNA that could act as an antisense RNA downregulating the expression of <em>galU</em>, the coding gene of uridine triphosphate-glucose-1-phosphate uridylyltransferase, and attenuating bacterial motility. <em>galU</em>, which is located at the opposite strand of <em>hns</em>, was identified to have a long 3′-untranslated region that overlapped with <em>hns</em> and could be processed to produce short RNA fragments. The overexpression of <em>hns</em> mRNA inhibited the expression of <em>galU</em>. The deletion of <em>galU</em> attenuated the motility of <em>S.</em> Typhi, while the complementation of <em>galU</em> nearly restored the phenotype. Overexpressing <em>hns</em> mRNA in the wild-type strain of <em>S.</em> Typhi inhibited the motility and the expression of flagellar genes, while overexpressing <em>hns</em> mRNA in the <em>galU</em>-deletion mutant did not influence bacterial motility. In conclusion, <em>hns</em> mRNA has been identified to be a new bifunctional RNA that attenuates the motility of <em>S.</em> Typhi by downregulating the expression of <em>galU</em>.</p></div>\",\"PeriodicalId\":50312,\"journal\":{\"name\":\"International Journal of Medical Microbiology\",\"volume\":\"311 6\",\"pages\":\"Article 151525\"},\"PeriodicalIF\":4.5000,\"publicationDate\":\"2021-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.ijmm.2021.151525\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Medical Microbiology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1438422121000540\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Medical Microbiology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1438422121000540","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
hns mRNA downregulates the expression of galU and attenuates the motility of Salmonella enterica serovar Typhi
Recently, multiple bifunctional RNAs have been discovered, which can both be translated into proteins and play regulatory roles. hns encodes the global gene silencing factor H-NS, which is widespread in Gram-negative bacteria. This study reported that hns mRNA of Salmonella enterica serovar Typhi (S. Typhi) was a bifunctional RNA that could act as an antisense RNA downregulating the expression of galU, the coding gene of uridine triphosphate-glucose-1-phosphate uridylyltransferase, and attenuating bacterial motility. galU, which is located at the opposite strand of hns, was identified to have a long 3′-untranslated region that overlapped with hns and could be processed to produce short RNA fragments. The overexpression of hns mRNA inhibited the expression of galU. The deletion of galU attenuated the motility of S. Typhi, while the complementation of galU nearly restored the phenotype. Overexpressing hns mRNA in the wild-type strain of S. Typhi inhibited the motility and the expression of flagellar genes, while overexpressing hns mRNA in the galU-deletion mutant did not influence bacterial motility. In conclusion, hns mRNA has been identified to be a new bifunctional RNA that attenuates the motility of S. Typhi by downregulating the expression of galU.
期刊介绍:
Pathogen genome sequencing projects have provided a wealth of data that need to be set in context to pathogenicity and the outcome of infections. In addition, the interplay between a pathogen and its host cell has become increasingly important to understand and interfere with diseases caused by microbial pathogens. IJMM meets these needs by focussing on genome and proteome analyses, studies dealing with the molecular mechanisms of pathogenicity and the evolution of pathogenic agents, the interactions between pathogens and host cells ("cellular microbiology"), and molecular epidemiology. To help the reader keeping up with the rapidly evolving new findings in the field of medical microbiology, IJMM publishes original articles, case studies and topical, state-of-the-art mini-reviews in a well balanced fashion. All articles are strictly peer-reviewed. Important topics are reinforced by 2 special issues per year dedicated to a particular theme. Finally, at irregular intervals, current opinions on recent or future developments in medical microbiology are presented in an editorial section.