Xiaohua Su, Zhidong Li, Bayaer Nashun, Juncheng Deng, Min Yue, Ming Lv, Weiwang Gu
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Establishment of a diabetes susceptible family in the Bama minipig (BMP) by N-ethyl-N-nitrosourea (ENU) induction.
Background: Diabetes mellitus (DM), characterized by hyperglycemia, is one of the leading causes of death worldwide. An appropriate DM animal model to explore the underlying mechanism of DM and develop new antidiabetic drugs is still desirable. Here, we aim to provide alternatives of DM animal models for medical researches.
Objective: To establish a diabetes susceptible family in the Bama minipig (BMP) by N-ethyl-N-nitrosourea (ENU) induction.
Methods: Male BMPs with hyperglycemia were selected from G1 and bred by the inbreeding strategy. After 5 generations, parameters such as fasting plasma glucose (FPG), 2-hour plasma glucose (2hPG), intravenous glucose-tolerance test (IVGTT), and insulin resistance were determined to evaluate susceptible family members.
Results: The male BMP 2907 (FPG = 6.1 mmol/L, IGVTT 2hPG = 11.9 mmol/L) with hyperglycemia was selected from G1 to generate the 2907 hyperglycemic family. With the number of breeding generations, average FPG levels in BMPs increased significantly (p < 0.05). G5 displayed the characteristics of elevated FPG, insulin resistance, dyslipidemia and abnormal glucose tolerance (p < 0.05).
Conclusion: A diabetes susceptible family has been successfully established, which might be used for further inbreeding or induced to mimic the phenotype of diabetes.
期刊介绍：
Neuroendocrinology Letters is an international, peer-reviewed interdisciplinary journal covering the fields of Neuroendocrinology, Neuroscience, Neurophysiology, Neuropsychopharmacology, Psychoneuroimmunology, Reproductive Medicine, Chronobiology, Human Ethology and related fields for RAPID publication of Original Papers, Review Articles, State-of-the-art, Clinical Reports and other contributions from all the fields covered by Neuroendocrinology
Letters.
Papers from both basic research (methodology, molecular and cellular biology, anatomy, histology, biology, embryology, teratology, normal and pathological physiology, biophysics, pharmacology, pathology and experimental pathology, biochemistry, neurochemistry, enzymology, chronobiology, receptor studies, endocrinology, immunology and neuroimmunology, animal physiology, animal breeding and ethology, human ethology, psychology and others) and from clinical research (neurology, psychiatry and child psychiatry, obstetrics and gynecology, pediatrics, endocrinology, immunology, cardiovascular studies, internal medicine, oncology and others) will be considered.
The Journal publishes Original papers and Review Articles. Brief reports, Special Communications, proved they are based on adequate experimental evidence, Clinical Studies, Case Reports, Commentaries, Discussions, Letters to the Editor (correspondence column), Book Reviews, Congress Reports and other categories of articles (philosophy, art, social issues, medical and health policies, biomedical history, etc.) will be taken under consideration.
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