人类患者分离的含vapn的马红球菌的致病性和基因组特征

IF 4.5 3区 医学 Q1 MICROBIOLOGY
Yasunori Suzuki , Hiroaki Kubota , Hiroo Madarame , Fumiaki Takase , Kei Takahashi , Yukako Sasaki , Tsutomu Kakuda , Shinji Takai
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引用次数: 12

摘要

马红球菌是一种腐生土壤细菌和细胞内病原体,可引起马驹难治性化脓性肺炎,并已成为人畜共患疾病的致病原因。几项研究报道了由马氏鼠携带最近描述的第三种毒力质粒引起的人类感染,即反刍动物相关的pVAPN,它携带vapN毒力决定因素。在此,我们分析了从人类HIV/AIDS患者和非HIV/AIDS患者中分离到的9株携带vapn的马鼠的致病性和基因组特征。其中4株在培养的巨噬细胞中表现出显著的VapN生成和增殖。这些菌株在小鼠中接种1.0 × 108 CFU后具有致死性致病性,并在肝脏和脾脏中产生与人类相似的坏死性肉芽肿性炎症。此外,我们确定了所有9株菌株的全基因组序列。序列长度为5.0 ~ 5.3 Mbp, GC含量为68.7% ~ 68.8%。所有菌株都有一个120或125 kbp的线性质粒携带vapN (I型或II型pVAPN),根据3 '侧远端序列的差异进行分类。有趣的是,在拥有几乎相同pVAPN类型的菌株之间,VapN的产生存在显著差异,差异仅限于几个snp和短碱基对索引。产生VapN的菌株所具有的pVAPN序列没有保留任何共同的遗传特征,需要更详细的分析,包括染色体基因,以进一步阐明VapN的表达机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Pathogenicity and genomic features of vapN-harboring Rhodococcus equi isolated from human patients

Rhodococcus equi is a saprophytic soil bacterium and intracellular pathogen that causes refractory suppurative pneumonia in foals and has emerged as a pathogenic cause of zoonotic disease. Several studies have reported human infections caused by R. equi harboring a recently described third type of virulence plasmid, the ruminant-associated pVAPN, which carries the vapN virulence determinant. Herein, we analyzed pathogenicity and genomic features of nine vapN-harboring R. equi isolated from human patients with and without HIV/AIDS. Four of these strains showed significant VapN production and proliferation in cultured macrophages. These strains were lethally pathogenic after inoculation with 1.0 × 108 CFU in mice and reproduced a necrotizing granulomatous inflammation in the liver and spleen similar to that observed in humans. Additionally, we determined entire genome sequences of all nine strains. Lengths of sequences were 5.0–5.3 Mbp, and GC contents were 68.7 %–68.8 %. All strains harbored a 120- or 125-kbp linear plasmid carrying vapN (Type I or Type II pVAPN) classified on the basis of differences in the distal sequences on the 3′ side. Interestingly, VapN production differed significantly among strains harboring nearly identical types of pVAPN with variation limited to several SNPs and short base pair indels. The pVAPN sequences possessed by the VapN-producing strains did not retain any common genetic characteristics, and more detailed analyses, including chromosomal genes, are needed to further elucidate the VapN expression mechanism.

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来源期刊
CiteScore
9.70
自引率
0.00%
发文量
18
审稿时长
45 days
期刊介绍: Pathogen genome sequencing projects have provided a wealth of data that need to be set in context to pathogenicity and the outcome of infections. In addition, the interplay between a pathogen and its host cell has become increasingly important to understand and interfere with diseases caused by microbial pathogens. IJMM meets these needs by focussing on genome and proteome analyses, studies dealing with the molecular mechanisms of pathogenicity and the evolution of pathogenic agents, the interactions between pathogens and host cells ("cellular microbiology"), and molecular epidemiology. To help the reader keeping up with the rapidly evolving new findings in the field of medical microbiology, IJMM publishes original articles, case studies and topical, state-of-the-art mini-reviews in a well balanced fashion. All articles are strictly peer-reviewed. Important topics are reinforced by 2 special issues per year dedicated to a particular theme. Finally, at irregular intervals, current opinions on recent or future developments in medical microbiology are presented in an editorial section.
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