引起急性尿路感染和无症状细菌性尿症的B群链球菌临床分离株的溶血活性和生物膜形成

IF 4.5 3区 医学 Q1 MICROBIOLOGY
Devika Desai , Kelvin G.K. Goh , Matthew J. Sullivan , Debasish Chattopadhyay , Glen C. Ulett
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引用次数: 4

摘要

无乳链球菌,又称B群链球菌,是成人尿路感染(UTI)的病原,包括膀胱炎、肾盂肾炎和无症状细菌性尿症(ABU)。尽管与尿源性无乳链球菌(UPSA)相比,由abu引起的无乳链球菌(ABSA)已被证明在人尿中生长并达到更高的培养密度,但从不同形式的UTI分离的无乳链球菌之间的表型差异尚不清楚。在这里,我们定义了一组临床分离的UPSA、ABSA和复发性无乳链球菌尿(rSAB)菌株的溶血活性和生物膜形成,以在分离株的临床历史背景下探索这些表型。采用血琼脂斑点法分析了61株UPSA、184株ABSA和47株rSAB的相对溶血活性,并用红细胞溶解悬浮液法对其进行了验证。采用Lysogeny微滴板法测定生物膜的形成,Todd-Hewitt培养基中添加1%葡萄糖诱导生物膜的形成。我们还使用多重PCR分析分离物是否存在编码粘连菌毛的基因,粘连菌毛有助于生物膜的形成。比较292株菌株的溶血活性,令人惊讶的是,与其他菌株相比,ABSA菌株明显更有可能高度溶血。相反,不同临床病史组菌株形成生物膜的相对能力没有差异。综上所述,这些发现表明无乳链球菌导致ABU具有高度溶血的倾向,但UTI菌株的临床病史与形成生物膜的能力之间没有联系。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Hemolytic activity and biofilm-formation among clinical isolates of group B streptococcus causing acute urinary tract infection and asymptomatic bacteriuria

Streptococcus agalactiae, also known as group B Streptococcus, is an aetiological agent of urinary tract infection (UTI) in adults, including cystitis, pyelonephritis and asymptomatic bacteriuria (ABU). Whereas ABU-causing S. agalactiae (ABSA) have been shown to grow and achieve higher culture denstity in human urine compared to uropathogenic S. agalactiae (UPSA) other phenotypic distinctions between S. agalactiae isolated from different forms of UTI are not known. Here, we define the hemolytic activities and biofilm-formation of a collection of clinical isolates of UPSA, ABSA and recurrent S. agalactiae bacteriuria (rSAB) strains to explore these phenotypes in the context of clinical history of isolates. A total of 61 UPSA, 184 ABSA, and 47 rSAB isolates were analyzed for relative hemolytic activity by spot assay on blood agar, which was validated using a erythrocyte lysis suspension assay. Biofilm formation was determined by microtiter plate assay with Lysogeny and Todd-Hewitt broths supplemented with 1% glucose to induce biofilm formation. We also used multiplex PCR to analyze isolates for the presence of genes encoding adhesive pili, which contribute to biofilm formation. Comparing the hemolytic activities of 292 isolates showed, surprisingly, that ABSA strains were significantly more likely to be highly hemolytic compared to other strains. In contrast, there were no differences between the relative abilities of strains from the different clinical history groups to form biofilms. Taken together, these findings demonstrate a propensity of S. agalactiae causing ABU to be highly hemolytic but no link between clinical history of UTI strains and ability to form biofilm.

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来源期刊
CiteScore
9.70
自引率
0.00%
发文量
18
审稿时长
45 days
期刊介绍: Pathogen genome sequencing projects have provided a wealth of data that need to be set in context to pathogenicity and the outcome of infections. In addition, the interplay between a pathogen and its host cell has become increasingly important to understand and interfere with diseases caused by microbial pathogens. IJMM meets these needs by focussing on genome and proteome analyses, studies dealing with the molecular mechanisms of pathogenicity and the evolution of pathogenic agents, the interactions between pathogens and host cells ("cellular microbiology"), and molecular epidemiology. To help the reader keeping up with the rapidly evolving new findings in the field of medical microbiology, IJMM publishes original articles, case studies and topical, state-of-the-art mini-reviews in a well balanced fashion. All articles are strictly peer-reviewed. Important topics are reinforced by 2 special issues per year dedicated to a particular theme. Finally, at irregular intervals, current opinions on recent or future developments in medical microbiology are presented in an editorial section.
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