全身性细菌感染影响树突状细胞的发育和功能

IF 4.5 3区 医学 Q1 MICROBIOLOGY
Kristin Bieber , Manina Günter , Karina A. Pasquevich , Stella E. Autenrieth
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引用次数: 3

摘要

树突状细胞(dc)是宿主防御感染的关键。DC耗竭是败血症过程中的早期事件,可能损害宿主的防御机制。在这里,我们研究了DC消耗和功能障碍是否与病原体无关,是否通过模式识别受体介导,是否由于革兰氏阴性细菌大肠杆菌和革兰氏阳性病原体金黄色葡萄球菌的全身感染而导致DC发育受损。大肠杆菌和金黄色葡萄球菌感染导致脾脏DC亚群和骨髓DC祖细胞(BM)的数量减少,金黄色葡萄球菌感染小鼠的这种影响持续时间明显长于大肠杆菌感染小鼠。DC亚群及其祖细胞的减少主要是与tlr无关的,感染诱导的单核细胞也是如此。此外,金黄色葡萄球菌感染小鼠的新生DC发育受损,大肠杆菌或金黄色葡萄球菌感染小鼠的BM细胞在体外有利于巨噬细胞分化。与大肠杆菌感染小鼠相比,由于DC数量减少和MHC II表达减少,在金黄色葡萄球菌中可以检测到CD4+和CD8+ T细胞,特别是产生Th1和IFN-γ的CD8+ T细胞。这些差异反映在大肠杆菌的快速杀伤,而金黄色葡萄球菌的细菌负荷增加。总之,我们的研究支持这样的观点,即全体性细菌感染通常会影响dc的数量和发展,从而影响T细胞反应,但其程度依赖于病原体。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Systemic bacterial infections affect dendritic cell development and function

Dendritic cells (DCs) are critical in host defense against infection. DC depletion is an early event in the course of sepsis that may impair the host defense mechanisms. Here, we addressed whether DC depletion and dysfunction are pathogen-independent, mediated via pattern recognition receptors, and are due to impaired DC development upon systemic infection with the Gram-negative bacterium Escherichia coli and the Gram-positive pathogen Staphylococcus aureus.

Infection with E. coli and S. aureus led to reduced numbers of splenic DC subsets and of DC progenitors in the bone marrow (BM) with this effect persisting significantly longer in mice infected with S. aureus than with E. coli. The reduction of DC subsets and their progenitors was mainly TLR-independent as was the infection-induced monopoiesis. Moreover, de novo DC development was impaired in mice infected with S. aureus, and BM cells from E. coli or S. aureus infected mice favored macrophage differentiation in vitro. As a consequence of reduced DC numbers and their reduced expression of MHC II less CD4+ and CD8+ T cells, especially Th1 and IFN-γ producing CD8+ T cells, could be detected in S. aureus compared to E. coli infected mice. These differences are reflected in the rapid killing of E. coli as opposed to an increase in bacterial load in S. aureus.

In summary, our study supports the idea that systemic bacterial infections generally affect the number and development of DCs and thereby the T cell responses, but the magnitude is pathogen-dependent.

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来源期刊
CiteScore
9.70
自引率
0.00%
发文量
18
审稿时长
45 days
期刊介绍: Pathogen genome sequencing projects have provided a wealth of data that need to be set in context to pathogenicity and the outcome of infections. In addition, the interplay between a pathogen and its host cell has become increasingly important to understand and interfere with diseases caused by microbial pathogens. IJMM meets these needs by focussing on genome and proteome analyses, studies dealing with the molecular mechanisms of pathogenicity and the evolution of pathogenic agents, the interactions between pathogens and host cells ("cellular microbiology"), and molecular epidemiology. To help the reader keeping up with the rapidly evolving new findings in the field of medical microbiology, IJMM publishes original articles, case studies and topical, state-of-the-art mini-reviews in a well balanced fashion. All articles are strictly peer-reviewed. Important topics are reinforced by 2 special issues per year dedicated to a particular theme. Finally, at irregular intervals, current opinions on recent or future developments in medical microbiology are presented in an editorial section.
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