COVID-19患者外周血淋巴细胞亚群的差异改变:双阳性和双阴性T细胞的上调

IF 2.3
Multidisciplinary Respiratory Medicine Pub Date : 2021-06-10 eCollection Date: 2021-01-15 DOI:10.4081/mrm.2021.758
Asmaa M Zahran, Zeinab Albadry M Zahran, Yasmeen H Mady, Essam Eldeen M O Mahran, Alaa Rashad, Ahmed Makboul, Khalid A Nasif, Aida A Abdelmaksoud, Omnia El-Badawy
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引用次数: 6

摘要

背景:病毒感染引起淋巴细胞总数及其亚群分布的改变。我们旨在研究COVID-19患者的外周血淋巴细胞亚群,并将这些亚群与临床和实验室数据联系起来,这可能有助于阐明COVID-19的发病机制,开发新的诊断和预后生物标志物。方法:对26例经反转录聚合酶链反应(RT-PCR)确诊的新冠肺炎患者进行病史采集和临床彻底检查。实验室检查包括全血细胞计数、D二聚体、铁蛋白和c反应蛋白(CRP)。胸部CT诊断COVID-19肺炎。用流式细胞术比较20名健康对照者的淋巴细胞亚群。结果:新冠肺炎患者白细胞减少、相对中性粒细胞减少、淋巴细胞减少、嗜酸性粒细胞减少,中性粒细胞/淋巴细胞比值明显升高。T细胞(包括CD4和CD8细胞、自然杀伤细胞(NK)和自然杀伤T细胞(NKT))明显减少。患者的双阳性T细胞(DPT)、双阴性T细胞(DNT)和B细胞相对于其他淋巴细胞亚群升高。结论:免疫炎症参数对了解新冠肺炎的发病机制和初步诊断具有重要意义。然而,在解释它们时必须足够小心,因为即使在同一地点的研究之间也观察到巨大的差异。B细胞、DPT细胞和DNT细胞在COVID-19发病和控制中的作用有待进一步研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Differential alterations in peripheral lymphocyte subsets in COVID-19 patients: upregulation of double-positive and double-negative T cells.

Differential alterations in peripheral lymphocyte subsets in COVID-19 patients: upregulation of double-positive and double-negative T cells.

Background: Viral infections cause alteration in the total number of lymphocytes and their subset distribution. We aimed to study peripheral blood lymphocyte subsets in COVID-19 patients and to correlate these subsets with clinical and laboratory data, which may help in clarifying the pathogenesis to develop novel diagnostic and prognostic biomarkers for COVID-19.

Methods: Twenty-six reverse-transcription polymerase chain reaction (RT-PCR) confirmed COVID-19 patients were subjected to medical history-taking and a thorough clinical examination. Laboratory tests included complete blood count, D dimer, ferritin, and C-reactive protein (CRP). Chest CT was used to diagnose COVID-19 pneumonia. Lymphocyte subsets were compared with those in 20 healthy controls using flow cytometry.

Results: Leucopenia, relative neutrophilia, lymphopenia, eosinopenia together with marked elevation in neutrophil/lymphocyte ratio were observed in our COVID-19 patients. A marked reduction was observed in T cells, including both CD4 and CD8 cells, natural killer (NK), and natural killer T cells (NKT). Double-positive T (DPT) cells, double-negative T (DNT) cells, and B cells were elevated in the patients relative to the other lymphocyte subsets.

Conclusion: Immune-inflammatory parameters are of utmost importance in understanding the pathogenesis and in the provisional diagnosis of COVID-19. Yet, adequate care must be taken during their interpretation because of the vast discrepancies observed between studies even in the same locality. Further studies are needed to clarify the role of B cells, DPT, and DNT cells in the pathogenesis and control of COVID-19.

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来源期刊
Multidisciplinary Respiratory Medicine
Multidisciplinary Respiratory Medicine Medicine-Pulmonary and Respiratory Medicine
自引率
0.00%
发文量
23
期刊介绍: Multidisciplinary Respiratory Medicine is the official journal of the Italian Respiratory Society - Società Italiana di Pneumologia (IRS/SIP). The journal publishes on all aspects of respiratory medicine and related fields, with a particular focus on interdisciplinary and translational research. The interdisciplinary nature of the journal provides a unique opportunity for researchers, clinicians and healthcare professionals across specialties to collaborate and exchange information. The journal provides a high visibility platform for the publication and dissemination of top quality original scientific articles, reviews and important position papers documenting clinical and experimental advances.
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