匹诺曹通过调节LACTB抑制结直肠癌细胞的增殖、迁移、侵袭和上皮间质转化。

Cancer biotherapy & radiopharmaceuticals Pub Date : 2022-09-01 Epub Date: 2020-12-31 DOI:10.1089/cbr.2020.4052
Lai Jiang, Yongbo Yang, Haiyang Feng, Qinfei Zhou, Yong Liu
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引用次数: 4

摘要

背景:结直肠癌是一种常见的消化道恶性肿瘤。匹诺曹蛋白(PINO)在结直肠癌中具有促凋亡作用。本研究旨在阐明PINO治疗如何影响结直肠癌细胞的其他生物学行为。材料与方法:通过不同浓度的PINO处理,检测PINO对HT29和HCT116细胞的影响。通过转染siRNA-LACTB来研究其在PINO治疗中的作用。采用细胞计数试剂盒-8法、伤口愈合法和Transwell法分别评价结直肠癌细胞的增殖、迁移和侵袭性。采用Western blot或定量逆转录-聚合酶链反应检测血清中LACTB、基质金属蛋白酶(MMP)-2、E-cadherin、N-cadherin的表达。结果:梯度PINO抑制结直肠癌细胞中MMP-2和N-cadherin的活性、迁移、侵袭性及表达,促进E-cadherin和LACTB的表达。沉默LACTB可促进结直肠癌细胞中MMP-2和N-cadherin的存活、迁移、侵袭和表达,抑制E-cadherin的表达。PINO抵消了沉默的LACTB的作用,而沉默的LACTB部分地消除了PINO对结直肠癌细胞的作用。结论:PINO通过调节LACTB抑制结直肠癌细胞的增殖、迁移、侵袭和上皮间质转化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Pinocembrin Inhibits the Proliferation, Migration, Invasiveness, and Epithelial-Mesenchymal Transition of Colorectal Cancer Cells by Regulating LACTB.

Background: Colorectal cancer (CRC) is a common malignancy of digestive tract. Pinocembrin (PINO) has been discovered to have a proapoptotic effect on CRC. This study aimed to elucidate how other biological behaviors of CRC cells were affected under PINO treatment. Materials and Methods: The effect of PINO on HT29 and HCT116 cells were detected through treatment of different concentrations of PINO. The role of LACTB in PINO treatment was investigated by transfection of siRNA-LACTB. Cell counting kit-8 assay, wound healing assay, and Transwell assay were conducted to evaluate the proliferation, migration, and invasiveness of CRC cells, respectively. Western blot or quantitative reverse transcription-polymerase chain reaction was carried out to measure the expressions of LACTB, matrix metalloproteinase (MMP)-2, E-cadherin, and N-cadherin. Results: Gradient PINO inhibited the viability, migration, invasiveness, and expressions of MMP-2 and N-cadherin in CRC cells, while promoted E-cadherin and LACTB expressions. Silencing LACTB promoted the viability, migration, invasiveness, and expressions of MMP-2 and N-cadherin in CRC cells and inhibited E-cadherin expression. PINO counteracted the effect of silenced LACTB, and yet silencing LACTB partially abolished the effect of PINO on CRC cells. Conclusion: PINO inhibited the proliferation, migration, invasiveness, and epithelial-to-mesenchymal transition of CRC cells by regulating LACTB.

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