病原体印迹有机硅氧烷聚合物作为选择性生物传感器检测目标大肠杆菌。

C Pub Date : 2018-05-14 DOI:10.3390/c4020029
Maria T Dulay, Naina Zaman, David Jaramillo, Alison C Mody, Richard N Zare
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引用次数: 0

摘要

病原体的早期检测需要快速、选择性强、灵敏度高且经济实惠的方法。我们报告了一种具有高灵敏度和高选择性的生物传感器的开发情况,这种传感器基于低成本制备的有机硅氧烷(OSX)聚合物,上面印有大肠杆菌-GFP(绿色荧光蛋白)。通过改变溶胶-凝胶反应的几个参数,制备出了具有高光学透明度、无裂纹和无收缩的 OSX 聚合物。通过复制压印法,将目标灭活大肠杆菌-GFP 的独特形状和化学指纹压印到大块聚合物中。在非层流条件下,对含有灭活大肠杆菌-GFP 的样品的捕获性能进行了研究,并与灭活鼠伤寒杆菌-GFP 进行了比较。捕获选择性比率取决于所使用的烷氧基硅烷类型、H2O:硅烷摩尔比和聚合温度。悬浮液中的细菌浓度范围为 ~6 × 105 到 1.6 × 109 cells/mL。含有聚乙二醇(PEG)的大肠杆菌压印 OSX 聚合物可区分目标细菌大肠杆菌、非目标细菌鼠伤寒杆菌和原生大肠杆菌-GFP,其选择性比率是聚二甲基硅氧烷(PDMS)和不含 PEG 的 OSX 聚合物的 4.5 倍。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Pathogen-Imprinted Organosiloxane Polymers as Selective Biosensors for the Detection of Targeted <i>E. coli</i>.

Pathogen-Imprinted Organosiloxane Polymers as Selective Biosensors for the Detection of Targeted <i>E. coli</i>.

Pathogen-Imprinted Organosiloxane Polymers as Selective Biosensors for the Detection of Targeted <i>E. coli</i>.

Pathogen-Imprinted Organosiloxane Polymers as Selective Biosensors for the Detection of Targeted E. coli.

Early detection of pathogens requires methods that are fast, selective, sensitive and affordable. We report the development of a biosensor with high sensitivity and selectivity based on the low-cost preparation of organosiloxane (OSX) polymers imprinted with E. coli-GFP (green fluorescent protein). OSX polymers with high optical transparency, no cracking, and no shrinkage were prepared by varying several parameters of the sol-gel reaction. The unique shape and chemical fingerprint of the targeted inactivated E. coli-GFP were imprinted into bulk polymers by replication imprinting where the polymer solution was dropcast onto a bacteria template that produced a replica of the bacterial shape and chemistry on the polymer surface upon removal of the template. Capture performances were studied under non-laminar flow conditions with samples containing inactivated E. coli-GFP and compared to inactivated S. typhimurium-GFP. Capture selectivity ratios are dependent on the type of alkoxysilanes used, the H2O:silane molar ratio, and the polymerization temperature. The bacteria concentration in suspension ranged from ~6 × 105 to 1.6 × 109 cells/mL. E. coli-imprinted OSX polymers with polyethylene glycol (PEG) differentiated between the targeted bacterium E. coli, and non-targeted bacteria S. typhimurium and native E. coli-GFP, achieving selectivity ratios up to 4.5 times higher than polydimethylsiloxane (PDMS) and OSX polymers without PEG.

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