Vesara A Gatera, Ronny Lesmana, Ida Musfiroh, Raden Tina D Judistiani, Budi Setiabudiawan, Rizky Abdulah
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引用次数: 0
摘要
背景 研究表明,肺部炎症会影响肺功能,甚至危及生命。维生素 D 可在抑制炎症细胞因子方面发挥重要作用。维生素 D 缺乏与多种肺部问题有关,包括呼吸窘迫综合征、肺泡炎症、上皮损伤和缺氧。很少有研究评估维生素 D 在预防肺泡细胞炎症方面的益处。材料与方法 我们开发了一种由脂多糖(LPS)处理诱导的细胞炎症模型。用 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四氮唑(MTT)检测维生素 D 对 LPS 诱导的 A549 细胞炎症的影响,并用 Western 印迹分析评估维生素 D 的抗炎机制。结果 我们的研究结果表明,维生素 D 可通过下调活化 B 细胞核因子卡巴轻链增强因子、肿瘤坏死因子-α、白细胞介素(IL)-1ß、IL-6 和 IL-12 促进 LPS 诱导的炎症后 A549 细胞的存活。结论 我们的研究结果表明,维生素 D 有可能控制肺部炎症,但还需要进一步研究。
Vitamin D Inhibits Lipopolysaccharide (LPS)-Induced Inflammation in A549 Cells by Downregulating Inflammatory Cytokines.
BACKGROUND Studies have shown that lung inflammation affects lung function, with life-threatening results. Vitamin D may play an important role in inhibiting inflammatory cytokines. Vitamin D deficiency is related to several lung problems, including respiratory distress syndrome, alveolar inflammation, epithelial damage, and hypoxia. Few studies have evaluated the benefits of vitamin D in preventing inflammation in alveolar cells. MATERIAL AND METHODS We developed a cell inflammation model induced by lipopolysaccharide (LPS) treatment. The effects of vitamin D on LPS-induced inflammation in A549 cells were examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and the anti-inflammatory mechanism of vitamin D was evaluated using western blot analysis. RESULTS Our results indicated that vitamin D promoted A549 cell survival following LPS-induced inflammation by downregulating nuclear factor nuclear factor kappa light chain enhancer of activated B cells, tumor necrosis factor-alpha, interleukin (IL)-1ß, IL-6, and IL-12. CONCLUSIONS Our results indicated that vitamin D has the potential to manage lung inflammation, although further studies are needed.