{"title":"伊朗养牛场家蝇种群敌敌畏抗性研究。","authors":"Ebrahim Ahmadi, Jahangir Khajehali","doi":"10.18502/jad.v14i4.5271","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Insecticide resistance is one of the most important problems associated with the control of <i>Musca domestica</i>, due to the potential of the rapid development of resistance to different chemical insecticides. The present study was carried out to evaluate dichlorvos resistance in the house fly populations collected from central regions of Iran, Isfahan Province and Chaharmahal and Bakhtiari Province, during 2017 to 2019.</p><p><strong>Methods: </strong>Bioassays were carried out using a standard topical application method as well as a fumigation method. The Koohrang population (susceptible) with the lowest LD<sub>50</sub> values to dichlorvos was chosen to calculate the resistance ratios (RR). Altered sensitivity of acetylcholinesterase (AChE), a target enzyme for dichlorvos, was investigated.</p><p><strong>Results: </strong>According to the results, very high levels of dichlorvos resistance were observed in the Mobarake population (RR= 80.25-fold by topical application and 33-fold by fumigation bioassay), and Isfahan population (RR= 107.30-fold by topical application and 43-fold by fumigation bioassay) compared to the Koohrang population. Acetylcholinesterase of the Koohrang population was the most sensitive to inhibition by dichlorvos based on the determination of median inhibitory concentration (IC<sub>50</sub>), but AChE of Mobarake and Isfahan populations were 741.93- and 343.94- fold less sensitive to inhibition.</p><p><strong>Conclusion: </strong>The insensitivity of AChE was possibly involved in dichlorvos resistance in the house fly populations.</p>","PeriodicalId":15095,"journal":{"name":"Journal of Arthropod-Borne Diseases","volume":"14 4","pages":"344-352"},"PeriodicalIF":0.6000,"publicationDate":"2020-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/9f/16/JAD-14-344.PMC8053071.pdf","citationCount":"4","resultStr":"{\"title\":\"Dichlorvos Resistance in the House Fly Populations, <i>Musca domestica</i>, of Iranian Cattle Farms.\",\"authors\":\"Ebrahim Ahmadi, Jahangir Khajehali\",\"doi\":\"10.18502/jad.v14i4.5271\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Insecticide resistance is one of the most important problems associated with the control of <i>Musca domestica</i>, due to the potential of the rapid development of resistance to different chemical insecticides. The present study was carried out to evaluate dichlorvos resistance in the house fly populations collected from central regions of Iran, Isfahan Province and Chaharmahal and Bakhtiari Province, during 2017 to 2019.</p><p><strong>Methods: </strong>Bioassays were carried out using a standard topical application method as well as a fumigation method. The Koohrang population (susceptible) with the lowest LD<sub>50</sub> values to dichlorvos was chosen to calculate the resistance ratios (RR). Altered sensitivity of acetylcholinesterase (AChE), a target enzyme for dichlorvos, was investigated.</p><p><strong>Results: </strong>According to the results, very high levels of dichlorvos resistance were observed in the Mobarake population (RR= 80.25-fold by topical application and 33-fold by fumigation bioassay), and Isfahan population (RR= 107.30-fold by topical application and 43-fold by fumigation bioassay) compared to the Koohrang population. Acetylcholinesterase of the Koohrang population was the most sensitive to inhibition by dichlorvos based on the determination of median inhibitory concentration (IC<sub>50</sub>), but AChE of Mobarake and Isfahan populations were 741.93- and 343.94- fold less sensitive to inhibition.</p><p><strong>Conclusion: </strong>The insensitivity of AChE was possibly involved in dichlorvos resistance in the house fly populations.</p>\",\"PeriodicalId\":15095,\"journal\":{\"name\":\"Journal of Arthropod-Borne Diseases\",\"volume\":\"14 4\",\"pages\":\"344-352\"},\"PeriodicalIF\":0.6000,\"publicationDate\":\"2020-12-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/9f/16/JAD-14-344.PMC8053071.pdf\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Arthropod-Borne Diseases\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.18502/jad.v14i4.5271\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2020/12/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q4\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Arthropod-Borne Diseases","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.18502/jad.v14i4.5271","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2020/12/1 0:00:00","PubModel":"eCollection","JCR":"Q4","JCRName":"PARASITOLOGY","Score":null,"Total":0}
Dichlorvos Resistance in the House Fly Populations, Musca domestica, of Iranian Cattle Farms.
Background: Insecticide resistance is one of the most important problems associated with the control of Musca domestica, due to the potential of the rapid development of resistance to different chemical insecticides. The present study was carried out to evaluate dichlorvos resistance in the house fly populations collected from central regions of Iran, Isfahan Province and Chaharmahal and Bakhtiari Province, during 2017 to 2019.
Methods: Bioassays were carried out using a standard topical application method as well as a fumigation method. The Koohrang population (susceptible) with the lowest LD50 values to dichlorvos was chosen to calculate the resistance ratios (RR). Altered sensitivity of acetylcholinesterase (AChE), a target enzyme for dichlorvos, was investigated.
Results: According to the results, very high levels of dichlorvos resistance were observed in the Mobarake population (RR= 80.25-fold by topical application and 33-fold by fumigation bioassay), and Isfahan population (RR= 107.30-fold by topical application and 43-fold by fumigation bioassay) compared to the Koohrang population. Acetylcholinesterase of the Koohrang population was the most sensitive to inhibition by dichlorvos based on the determination of median inhibitory concentration (IC50), but AChE of Mobarake and Isfahan populations were 741.93- and 343.94- fold less sensitive to inhibition.
Conclusion: The insensitivity of AChE was possibly involved in dichlorvos resistance in the house fly populations.
期刊介绍：
The journal publishes original research paper, short communication, scientific note, case report, letter to the editor, and review article in English. The scope of papers comprises all aspects of arthropod borne diseases including:
● Systematics
● Vector ecology
● Epidemiology
● Immunology
● Parasitology
● Molecular biology
● Genetics
● Population dynamics
● Toxicology
● Vector control
● Diagnosis and treatment and other related subjects.
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