Le Wang, Wenbo Diwu, Nana Tan, Huan Wang, Jingbo Hu, Bailu Xu, Xiaoling Wang
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引用次数: 2
摘要
天然产物已广泛应用于2型糖尿病(T2D)的治疗。然而,由于其多组分、多靶点的特点,其作用机制往往模糊不清。作者构建了黄芩(Scutellaria baicalensis Georgi, SBG)中13种α-葡萄糖苷酶抑制剂(AGIs)靶蛋白的pathway- protein association (PPA)网络,旨在探讨其作用机制。该网络包含118个节点和1167个连接。聚类系数高,平均路径长度短,分布程度不均匀,具有小世界特性。PPA网络具有固有的层次结构,C(k) ~ k−0.71。同时还表现出潜在的弱失配混合模式,并伴有函数Knn (k)的减小和配度系数的负值。这些特性表明,一些节点对网络至关重要。然后将PGH2、GNAS、MAPK1、MAPK3、PRKCA和MAOA确定为度值和中心性指数最高的关键靶点。此外,一个核心子网络显示,黄菊花素、5,8,2 ' -三羟基-7-甲氧基黄酮和黄酮素是这些AGIs的主要活性成分,血清素能突触途径是SBG抗T2D的关键途径。基于通路的蛋白-蛋白关联网络的应用为探索天然产物治疗复杂疾病的机制提供了一种新的策略。
Pathway-based protein–protein association network to explore mechanism of α-glucosidase inhibitors from Scutellaria baicalensis Georgi against type 2 diabetes
Natural products have been widely used in the treatment of type 2 diabetes (T2D). However, their mechanisms are often obscured due to multi-components and multi-targets. The authors constructed a pathway-based protein–protein association (PPA) network for target proteins of 13 α-glucosidase inhibitors (AGIs) identified from Scutellaria baicalensis Georgi (SBG), designed to explore the underlying mechanisms. This network contained 118 nodes and 1167 connections. An uneven degree distribution and small-world property were observed, characterised by high clustering coefficient and short average path length. The PPA network had an inherent hierarchy as C(k)∼k−0.71. It also exhibited potential weak disassortative mixing pattern, coupled with a decreased function Knn (k) and negative value of assortativity coefficient. These properties indicated that a few nodes were crucial to the network. PGH2, GNAS, MAPK1, MAPK3, PRKCA, and MAOA were then identified as key targets with the highest degree values and centrality indices. Additionally, a core subnetwork showed that chrysin, 5,8,2′-trihydroxy-7-methoxyflavone, and wogonin were the main active constituents of these AGIs, and that the serotonergic synapse pathway was the critical pathway for SBG against T2D. The application of a pathway-based protein–protein association network provides a novel strategy to explore the mechanisms of natural products on complex diseases.
期刊介绍:
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.