自噬与乳腺癌亚型中一个强大的特异性转录特征相关。

Q2 Biochemistry, Genetics and Molecular Biology
Genes and Cancer Pub Date : 2020-10-06 eCollection Date: 2020-12-31 DOI:10.18632/genesandcancer.208
Céline Grandvallet, Jean Paul Feugeas, Franck Monnien, Gilles Despouy, Perez Valérie, Guittaut Michaël, Eric Hervouet, Paul Peixoto
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引用次数: 7

摘要

先前的研究已经描述了自噬可能与促癌和抗癌特性相关,这取决于许多因素,例如所考虑的基因、涉及的自噬步骤或所使用的癌症模型。这些数据可能是由于一些自噬相关基因可能参与其他细胞过程,因此根据肿瘤的类型或等级不同而受到不同的调节。事实上,使用不同的乳腺癌转录组分析方法,并使用数字PCR进一步确认,我们确定了与Luminal a或三阴性乳腺癌(TNBC)相关的自噬基因表达的特定特征。此外,我们证实,在TNBC中特异性表达的自噬基因ATG5有利于细胞迁移,而与er阳性乳腺癌特异性相关的自噬基因BECN1则诱导相反的作用。我们还发现,对自噬的整体抑制促进了细胞迁移,这表明单个ATG基因在癌症表型中的作用并不严格依赖于它们在自噬过程中的功能。最后,我们的工作导致了TXNIP1作为乳腺癌自噬诱导相关的潜在生物标志物的鉴定。该基因可以成为量化固定活检中自噬水平的重要工具,根据自噬水平对肿瘤进行分类,并确定最佳治疗方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Autophagy is associated with a robust specific transcriptional signature in breast cancer subtypes.

Autophagy is associated with a robust specific transcriptional signature in breast cancer subtypes.

Autophagy is associated with a robust specific transcriptional signature in breast cancer subtypes.

Autophagy is associated with a robust specific transcriptional signature in breast cancer subtypes.

Previous works have described that autophagy could be associated to both pro- and anti-cancer properties according to numerous factors, such as the gene considered, the step of autophagy involved or the cancer model used. These data might be explained by the fact that some autophagy-related genes may be involved in other cellular processes and therefore differently regulated according to the type or the grade of the tumor. Indeed, using different approaches of transcriptome analysis in breast cancers, and further confirmation using digital PCR, we identified a specific signature of autophagy gene expression associated to Luminal A or Triple Negative Breast Cancers (TNBC). Moreover, we confirmed that ATG5, an autophagy gene specifically expressed in TNBC, favored cell migration, whereas BECN1, an autophagy gene specifically associated with ER-positive breast cancers, induced opposite effects. We also showed that overall inhibition of autophagy promoted cell migration suggesting that the role of individual ATG genes in cancer phenotypes was not strictly dependent of their function during autophagy. Finally, our work led to the identification of TXNIP1 as a potential biomarker associated to autophagy induction in breast cancers. This gene could become an essential tool to quantify autophagy levels in fixed biopsies, sort tumors according to their autophagy levels and determine the best therapeutic treatment.

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来源期刊
Genes and Cancer
Genes and Cancer Biochemistry, Genetics and Molecular Biology-Genetics
CiteScore
3.90
自引率
0.00%
发文量
6
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