转座子诱导的逆转录激活玉米果皮中的基因表达。

IF 3.3 3区 生物学
Genetics Pub Date : 2021-06-24 DOI:10.1093/genetics/iyab062
Sharu Paul Sharma, Tao Zuo, Thomas Peterson
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引用次数: 3

摘要

染色体反转可以产生相当大的生物学和农学影响,包括破坏基因功能、改变基因表达和抑制重组。在这里,我们描述了由负责玉米花色素沉着的p1和p2基因之间的选择性易位引起的六个反转的分子结构和功能影响。在玉米品系p1-wwB54中,p1基因为空,p2基因在花药和丝中表达,但在果皮中不表达,使玉米粒变白。通过筛选具有红色果皮的核,我们确定了由Ac和断裂的Ac(fAc)转座元件的转座引起的该区域的反转。我们假设这些反转将p2基因启动子置于p1基因增强子附近,从而激活核果皮中p2的表达。据我们所知,这是首次报道在真核生物中改变基因启动子相对于增强子的位置以诱导异位表达的多次重复性反转。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Transposon-induced inversions activate gene expression in the maize pericarp.

Transposon-induced inversions activate gene expression in the maize pericarp.

Transposon-induced inversions activate gene expression in the maize pericarp.

Transposon-induced inversions activate gene expression in the maize pericarp.

Chromosomal inversions can have considerable biological and agronomic impacts including disrupted gene function, change in gene expression, and inhibited recombination. Here, we describe the molecular structure and functional impact of six inversions caused by Alternative Transpositions between p1 and p2 genes responsible for floral pigmentation in maize. In maize line p1-wwB54, the p1 gene is null and the p2 gene is expressed in anther and silk but not in pericarp, making the kernels white. By screening for kernels with red pericarp, we identified inversions in this region caused by transposition of Ac and fractured Ac (fAc) transposable elements. We hypothesize that these inversions place the p2 gene promoter near a p1 gene enhancer, thereby activating p2 expression in kernel pericarp. To our knowledge, this is the first report of multiple recurrent inversions that change the position of a gene promoter relative to an enhancer to induce ectopic expression in a eukaryote.

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来源期刊
Genetics
Genetics 生物-遗传学
CiteScore
6.20
自引率
6.10%
发文量
177
期刊介绍: GENETICS is published by the Genetics Society of America, a scholarly society that seeks to deepen our understanding of the living world by advancing our understanding of genetics. Since 1916, GENETICS has published high-quality, original research presenting novel findings bearing on genetics and genomics. The journal publishes empirical studies of organisms ranging from microbes to humans, as well as theoretical work. While it has an illustrious history, GENETICS has changed along with the communities it serves: it is not your mentor''s journal. The editors make decisions quickly – in around 30 days – without sacrificing the excellence and scholarship for which the journal has long been known. GENETICS is a peer reviewed, peer-edited journal, with an international reach and increasing visibility and impact. All editorial decisions are made through collaboration of at least two editors who are practicing scientists. GENETICS is constantly innovating: expanded types of content include Reviews, Commentary (current issues of interest to geneticists), Perspectives (historical), Primers (to introduce primary literature into the classroom), Toolbox Reviews, plus YeastBook, FlyBook, and WormBook (coming spring 2016). For particularly time-sensitive results, we publish Communications. As part of our mission to serve our communities, we''ve published thematic collections, including Genomic Selection, Multiparental Populations, Mouse Collaborative Cross, and the Genetics of Sex.
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