Anna Szilasi, Lilla Dénes, Eszter Krikó, Caoimhe Murray, Míra Mándoki, Gyula Balka
{"title":"猫白血病病毒和猫免疫缺陷病毒在爱尔兰家猫中的流行。","authors":"Anna Szilasi, Lilla Dénes, Eszter Krikó, Caoimhe Murray, Míra Mándoki, Gyula Balka","doi":"10.1556/004.2020.00056","DOIUrl":null,"url":null,"abstract":"<p><p>Feline immunodeficiency virus (FIV) and feline leukaemia virus (FeLV) are retroviruses affecting felid species worldwide. A study was performed over a period of 5 months in Ireland with the aim to get an updated and more realistic prevalence of these retroviruses. A total of 183 EDTA-anticoagulated whole-blood samples were collected from cats distributed between 10 clinics. The samples were tested using both point-of-care enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR). Basic clinical data and vaccination history were also recorded for the sampled cats. The results of ELISA tests showed a prevalence of 10.4 and 3.3% for FIV and FeLV, respectively, and an apparent prevalence of 9.3% for FIV and 11.6% for FeLV with PCR. Phylogenetic analysis of the partial polymerase (pol) gene sequences obtained from 8 FIV-positive strains showed that all but one of the Irish strains belonged to FIV subtype A, and one to subtype B. The overall mean genetic similarity between the analysed strains was 91.15%.</p>","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":"68 4","pages":"413-420"},"PeriodicalIF":0.7000,"publicationDate":"2021-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"5","resultStr":"{\"title\":\"Prevalence of feline leukaemia virus and feline immunodeficiency virus in domestic cats in Ireland.\",\"authors\":\"Anna Szilasi, Lilla Dénes, Eszter Krikó, Caoimhe Murray, Míra Mándoki, Gyula Balka\",\"doi\":\"10.1556/004.2020.00056\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Feline immunodeficiency virus (FIV) and feline leukaemia virus (FeLV) are retroviruses affecting felid species worldwide. A study was performed over a period of 5 months in Ireland with the aim to get an updated and more realistic prevalence of these retroviruses. A total of 183 EDTA-anticoagulated whole-blood samples were collected from cats distributed between 10 clinics. The samples were tested using both point-of-care enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR). Basic clinical data and vaccination history were also recorded for the sampled cats. The results of ELISA tests showed a prevalence of 10.4 and 3.3% for FIV and FeLV, respectively, and an apparent prevalence of 9.3% for FIV and 11.6% for FeLV with PCR. Phylogenetic analysis of the partial polymerase (pol) gene sequences obtained from 8 FIV-positive strains showed that all but one of the Irish strains belonged to FIV subtype A, and one to subtype B. The overall mean genetic similarity between the analysed strains was 91.15%.</p>\",\"PeriodicalId\":7247,\"journal\":{\"name\":\"Acta veterinaria Hungarica\",\"volume\":\"68 4\",\"pages\":\"413-420\"},\"PeriodicalIF\":0.7000,\"publicationDate\":\"2021-01-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta veterinaria Hungarica\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1556/004.2020.00056\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta veterinaria Hungarica","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1556/004.2020.00056","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
Prevalence of feline leukaemia virus and feline immunodeficiency virus in domestic cats in Ireland.
Feline immunodeficiency virus (FIV) and feline leukaemia virus (FeLV) are retroviruses affecting felid species worldwide. A study was performed over a period of 5 months in Ireland with the aim to get an updated and more realistic prevalence of these retroviruses. A total of 183 EDTA-anticoagulated whole-blood samples were collected from cats distributed between 10 clinics. The samples were tested using both point-of-care enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR). Basic clinical data and vaccination history were also recorded for the sampled cats. The results of ELISA tests showed a prevalence of 10.4 and 3.3% for FIV and FeLV, respectively, and an apparent prevalence of 9.3% for FIV and 11.6% for FeLV with PCR. Phylogenetic analysis of the partial polymerase (pol) gene sequences obtained from 8 FIV-positive strains showed that all but one of the Irish strains belonged to FIV subtype A, and one to subtype B. The overall mean genetic similarity between the analysed strains was 91.15%.
期刊介绍:
Acta Veterinaria Hungarica publishes original research papers presenting new scientific results of international interest, and to a limited extent also review articles and clinical case reports, on veterinary physiology (physiological chemistry and metabolism), veterinary microbiology (bacteriology, virology, immunology, molecular biology), on the infectious diseases of domestic animals, on veterinary parasitology, pathology, clinical veterinary science and reproduction.