{"title":"干细胞治疗对脑卒中小鼠星形胶质细胞形成的影响。","authors":"Kobra Akhoundzadeh, Abedin Vakili","doi":"10.21037/sci-2020-031","DOIUrl":null,"url":null,"abstract":"<p><p>This study was planned to continue our previous study to assess effect of combination therapy bone marrow stromal cells (BMSCs) with exercise (EX) and triiodothyronine (T<sub>3</sub>) on stroke-induced astrogliosis in mice. Stroke subjected-mice were divided into five monotherapy groups including sham, control, BMSCs, EX and T<sub>3</sub>; and three combination therapy groups including BMSCs + EX, BMSCs + T<sub>3</sub> and BMSCs + EX + T<sub>3</sub>. Astrogliosis was assessed in ipsilateral hemisphere at day 7 after MCAO. Combination therapy BMSCs with EX and T<sub>3</sub> could significantly decrease stroke-induced astrogliosis. However, monotherapy with BMSCs or EX also improved changes of glial fibrillary acidic protein (GFAP)-positive cells following stroke. Combination therapy BMSCs with EX and T<sub>3</sub> didn't have any added effect on astrogliosis compared to monotherapy with BMSCs or EX. With comparing the present findings with the results of neurobehavioral functioning in our earlier study, it seems that decrease of astrogliosis could be helpful for stroke recovery.</p>","PeriodicalId":21938,"journal":{"name":"Stem cell investigation","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2020-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7791154/pdf/sci-07-2020-031.pdf","citationCount":"1","resultStr":"{\"title\":\"Effect of stem cells-based therapy on astrogliosis in stroke subjected-mice.\",\"authors\":\"Kobra Akhoundzadeh, Abedin Vakili\",\"doi\":\"10.21037/sci-2020-031\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>This study was planned to continue our previous study to assess effect of combination therapy bone marrow stromal cells (BMSCs) with exercise (EX) and triiodothyronine (T<sub>3</sub>) on stroke-induced astrogliosis in mice. Stroke subjected-mice were divided into five monotherapy groups including sham, control, BMSCs, EX and T<sub>3</sub>; and three combination therapy groups including BMSCs + EX, BMSCs + T<sub>3</sub> and BMSCs + EX + T<sub>3</sub>. Astrogliosis was assessed in ipsilateral hemisphere at day 7 after MCAO. Combination therapy BMSCs with EX and T<sub>3</sub> could significantly decrease stroke-induced astrogliosis. However, monotherapy with BMSCs or EX also improved changes of glial fibrillary acidic protein (GFAP)-positive cells following stroke. Combination therapy BMSCs with EX and T<sub>3</sub> didn't have any added effect on astrogliosis compared to monotherapy with BMSCs or EX. With comparing the present findings with the results of neurobehavioral functioning in our earlier study, it seems that decrease of astrogliosis could be helpful for stroke recovery.</p>\",\"PeriodicalId\":21938,\"journal\":{\"name\":\"Stem cell investigation\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-12-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7791154/pdf/sci-07-2020-031.pdf\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Stem cell investigation\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.21037/sci-2020-031\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2020/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q1\",\"JCRName\":\"Biochemistry, Genetics and Molecular Biology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Stem cell investigation","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21037/sci-2020-031","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2020/1/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 1
摘要
本研究计划继续我们先前的研究，以评估骨髓基质细胞(BMSCs)与运动(EX)和三碘甲状腺原氨酸(T3)联合治疗小鼠脑卒中诱导的星形胶质细胞形成的影响。将脑卒中小鼠分为假手术组、对照组、骨髓间充质干细胞组、EX组和T3组;BMSCs + EX、BMSCs + T3、BMSCs + EX + T3三个联合治疗组。MCAO术后第7天对同侧半球星形胶质细胞形成进行评估。BMSCs与EX和T3联合治疗可显著减少脑卒中诱导的星形胶质细胞增生。然而，骨髓间充质干细胞或EX单药治疗也能改善脑卒中后胶质纤维酸性蛋白(GFAP)阳性细胞的变化。与BMSCs或EX单药治疗相比，BMSCs与EX和T3联合治疗对星形胶质细胞增生没有任何额外的作用。将本研究结果与我们早期研究的神经行为功能结果进行比较，似乎星形胶质细胞增生的减少可能有助于中风的恢复。
Effect of stem cells-based therapy on astrogliosis in stroke subjected-mice.
This study was planned to continue our previous study to assess effect of combination therapy bone marrow stromal cells (BMSCs) with exercise (EX) and triiodothyronine (T3) on stroke-induced astrogliosis in mice. Stroke subjected-mice were divided into five monotherapy groups including sham, control, BMSCs, EX and T3; and three combination therapy groups including BMSCs + EX, BMSCs + T3 and BMSCs + EX + T3. Astrogliosis was assessed in ipsilateral hemisphere at day 7 after MCAO. Combination therapy BMSCs with EX and T3 could significantly decrease stroke-induced astrogliosis. However, monotherapy with BMSCs or EX also improved changes of glial fibrillary acidic protein (GFAP)-positive cells following stroke. Combination therapy BMSCs with EX and T3 didn't have any added effect on astrogliosis compared to monotherapy with BMSCs or EX. With comparing the present findings with the results of neurobehavioral functioning in our earlier study, it seems that decrease of astrogliosis could be helpful for stroke recovery.
期刊介绍：
The Stem Cell Investigation (SCI; Stem Cell Investig; Online ISSN: 2313-0792) is a free access, peer-reviewed online journal covering basic, translational, and clinical research on all aspects of stem cells. It publishes original research articles and reviews on embryonic stem cells, induced pluripotent stem cells, adult tissue-specific stem/progenitor cells, cancer stem like cells, stem cell niche, stem cell technology, stem cell based drug discovery, and regenerative medicine. Stem Cell Investigation is indexed in PubMed/PMC since April, 2016.
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