双壳贝类与众不同:一氧化氮是太平洋牡蛎(Crassostrea gigas)变态过程中的负调节因子。

Q2 Biochemistry, Genetics and Molecular Biology
Susanne Vogeler, Stefano Carboni, Xiaoxu Li, Nancy Nevejan, Sean J Monaghan, Jacqueline H Ireland, Alyssa Joyce
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引用次数: 0

摘要

背景:一氧化氮(NO)被认为是许多无脊椎动物变态的调控因子,尽管对腹足类、无脊椎动物和甲壳类的 NO 途径研究相对较多,但有关 NO 对双壳贝类变态影响的研究却非常有限:本文研究了一氧化氮途径抑制剂和一氧化氮供体对太平洋牡蛎(Crassostrea gigas)幼体变态诱导的影响。一氧化氮合酶(NOS)抑制剂 s-甲基异硫脲半硫酸盐(SMIS)、氨基胍半硫酸盐(AGH)和 7-硝基吲唑(7-NI)的变态诱导率分别为 75%、76% 和 83%,且其作用方式与浓度有关。1H-[1,2,4]噁二唑[4,3-a]喹喔啉-1-酮(ODQ)是一种可溶性鸟苷酸环化酶的抑制剂,NO 与之相互作用,催化环鸟苷酸单磷酸(cGMP)的合成。相反,高浓度的氮氧化物供体二水硝普钠与变态诱导剂肾上腺素、MK-801 或 SMIS 结合使用,会显著降低变态率,但不能排除与变态途径无关的过量氮氧化物的潜在有害影响。无论使用哪种诱导剂,幼虫变态后 CgNOS 的表达也会下降,但在吐丝变态后又会增强。用 DAF-FM 二乙酸盐荧光检测有能力幼体中的一氧化氮,并通过原位杂交确定牡蛎一氧化氮合酶 CgNOS 的表达位置,结果表明一氧化氮主要发生在两个关键的幼体结构中,即绒毛和足部:总之,这些结果表明,氮氧化物途径是太平洋牡蛎幼体变态的负向调节因子,氮氧化物的减少通过抑制游泳或爬行行为以及一系列额外的神经内分泌下游反应来诱导变态。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Bivalves are NO different: nitric oxide as negative regulator of metamorphosis in the Pacific oyster, Crassostrea gigas.

Bivalves are NO different: nitric oxide as negative regulator of metamorphosis in the Pacific oyster, Crassostrea gigas.

Bivalves are NO different: nitric oxide as negative regulator of metamorphosis in the Pacific oyster, Crassostrea gigas.

Bivalves are NO different: nitric oxide as negative regulator of metamorphosis in the Pacific oyster, Crassostrea gigas.

Background: Nitric oxide (NO) is presumed to be a regulator of metamorphosis in many invertebrate species, and although NO pathways have been comparatively well-investigated in gastropods, annelids and crustaceans, there has been very limited research on the effects of NO on metamorphosis in bivalve shellfish.

Results: In this paper, we investigate the effects of NO pathway inhibitors and NO donors on metamorphosis induction in larvae of the Pacific oyster, Crassostrea gigas. The nitric oxides synthase (NOS) inhibitors s-methylisothiourea hemisulfate salt (SMIS), aminoguanidine hemisulfate salt (AGH) and 7-nitroindazole (7-NI) induced metamorphosis at 75, 76 and 83% respectively, and operating in a concentration-dependent manner. Additional induction of up to 54% resulted from exposures to 1H-[1,2,4]Oxadiazole[4,3-a]quinoxalin-1-one (ODQ), an inhibitor of soluble guanylyl cyclase, with which NO interacts to catalyse the synthesis of cyclic guanosine monophosphate (cGMP). Conversely, high concentrations of the NO donor sodium nitroprusside dihydrate in combination with metamorphosis inducers epinephrine, MK-801 or SMIS, significantly decreased metamorphosis, although a potential harmful effect of excessive NO unrelated to metamorphosis pathway cannot be excluded. Expression of CgNOS also decreased in larvae after metamorphosis regardless of the inducers used, but intensified again post-metamorphosis in spat. Fluorescent detection of NO in competent larvae with DAF-FM diacetate and localisation of the oyster nitric oxide synthase CgNOS expression by in-situ hybridisation showed that NO occurs primarily in two key larval structures, the velum and foot. cGMP was also detected in the foot using immunofluorescent assays, and is potentially involved in the foot's smooth muscle relaxation.

Conclusion: Together, these results suggest that the NO pathway acts as a negative regulator of metamorphosis in Pacific oyster larvae, and that NO reduction induces metamorphosis by inhibiting swimming or crawling behaviour, in conjunction with a cascade of additional neuroendocrine downstream responses.

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来源期刊
BMC Developmental Biology
BMC Developmental Biology 生物-发育生物学
自引率
0.00%
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0
审稿时长
>12 weeks
期刊介绍: BMC Developmental Biology is an open access, peer-reviewed journal that considers articles on the development, growth, differentiation and regeneration of multicellular organisms, including molecular, cellular, tissue, organ and whole organism research.
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