阿根廷 Anastrepha fraterculus sp.

IF 2.9 Q2 Biochemistry, Genetics and Molecular Biology
María Cecilia Giardini, Mariela Nieves, Alejandra Carla Scannapieco, Claudia Alejandra Conte, Fabián Horacio Milla, María Elena Schapovaloff, Maria Soledad Frissolo, María Isabel Remis, Jorge Luis Cladera, Silvia Beatriz Lanzavecchia
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In this work, we expanded the cytological study of this morphotype by analyzing laboratory strains and wild populations to provide information about the frequency and geographic distribution of these sex chromosome variants. We analyzed the mitotic metaphases of individuals from four laboratory strains and five wild populations from the main fruit-producing areas of Argentina, including the northwest (Tucumán and La Rioja), northeast (Entre Ríos and Misiones), and center (Buenos Aires) of the country.</p><p><strong>Results: </strong>In wild samples, we observed a high frequency of X<sub>1</sub>X<sub>1</sub> (0.94) and X<sub>1</sub>Y<sub>5</sub> (0.93) karyomorphs, whereas X<sub>1</sub>X<sub>2</sub> and X<sub>1</sub>Y<sub>6</sub> were exclusively found at a low frequency in Buenos Aires (0.07 and 0.13, respectively), Entre Ríos (0.16 and 0.14, respectively) and Tucumán (0.03 and 0.04, respectively). 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引用次数: 0

摘要

背景:Anastrepha fraterculus 在多个美洲国家被认定为检疫害虫。这种果蝇原产于美洲大陆,分布于热带和亚热带地区。据报道,它是一个隐蔽种的复合体,至少有 8 个形态型被描述过。该复合体中只有一个实体存在于阿根廷,原名为 Anastrepha fraterculus sp.之前对这一形态型的细胞遗传学研究描述了性染色体变异的存在,通过染色体大小和染色模式进行了鉴定。在这项工作中,我们通过分析实验室菌株和野生种群,扩大了对这一形态的细胞学研究,以提供有关这些性染色体变异的频率和地理分布的信息。我们分析了来自阿根廷西北部(图库曼和拉里奥哈)、东北部(恩特雷里奥斯和米西奥内斯)和中部(布宜诺斯艾利斯)等主要水果产区的四个实验室品系和五个野生种群个体的有丝分裂分裂相:在野生样本中,我们发现 X1X1(0.94)和 X1Y5(0.93)核形体的频率较高,而 X1X2 和 X1Y6 则仅在布宜诺斯艾利斯(分别为 0.07 和 0.13)、恩特雷里奥斯(分别为 0.16 和 0.14)和图库曼(分别为 0.03 和 0.04)发现,频率较低。在野生种群中没有发现 X2X2 和 X2Y5 核型,但在实验室菌株中发现的频率较低。事实上,野生种群和实验室菌株的核仁频率是不同的。A.fraterculus野生种群的核型频率和染色体频率均无明显差异。然而,Y5染色体频率与纬度之间存在明显的相关性:我们讨论了细胞遗传学的重要性,以了解这种害虫在阿根廷可能的入侵和扩散路线,以及在实验室条件下可能驱动染色体频率变化的进化力量。我们的研究结果为该物种提供了深入而全面的遗传学知识,对大规模饲养生产和实施 SIT 所需的有价值的 A. fraterculus sp.
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Geographic distribution of sex chromosome polymorphism in Anastrepha fraterculus sp. 1 from Argentina.

Geographic distribution of sex chromosome polymorphism in Anastrepha fraterculus sp. 1 from Argentina.

Geographic distribution of sex chromosome polymorphism in Anastrepha fraterculus sp. 1 from Argentina.

Geographic distribution of sex chromosome polymorphism in Anastrepha fraterculus sp. 1 from Argentina.

Background: Anastrepha fraterculus is recognized as a quarantine pest in several American countries. This fruit fly species is native to the American continent and distributed throughout tropical and subtropical regions. It has been reported as a complex of cryptic species, and at least eight morphotypes have been described. Only one entity of this complex, formerly named Anastrepha fraterculus sp. 1, is present in Argentina. Previous cytogenetic studies on this morphotype described the presence of sex chromosome variation identified by chromosomal size and staining patterns. In this work, we expanded the cytological study of this morphotype by analyzing laboratory strains and wild populations to provide information about the frequency and geographic distribution of these sex chromosome variants. We analyzed the mitotic metaphases of individuals from four laboratory strains and five wild populations from the main fruit-producing areas of Argentina, including the northwest (Tucumán and La Rioja), northeast (Entre Ríos and Misiones), and center (Buenos Aires) of the country.

Results: In wild samples, we observed a high frequency of X1X1 (0.94) and X1Y5 (0.93) karyomorphs, whereas X1X2 and X1Y6 were exclusively found at a low frequency in Buenos Aires (0.07 and 0.13, respectively), Entre Ríos (0.16 and 0.14, respectively) and Tucumán (0.03 and 0.04, respectively). X2X2 and X2Y5 karyomorphs were not found in wild populations but were detected at a low frequency in laboratory strains. In fact, karyomorph frequencies differed between wild populations and laboratory strains. No significant differences among A. fraterculus wild populations were evidenced in either karyotypic or chromosomal frequencies. However, a significant correlation was observed between Y5 chromosomal frequency and latitude.

Conclusions: We discuss the importance of cytogenetics to understand the possible route of invasion and dispersion of this pest in Argentina and the evolutionary forces acting under laboratory conditions, possibly driving changes in the chromosomal frequencies. Our findings provide deep and integral genetic knowledge of this species, which has become of relevance to the characterization and selection of valuable A. fraterculus sp. 1 strains for mass rearing production and SIT implementation.

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来源期刊
BMC Genetics
BMC Genetics 生物-遗传学
CiteScore
4.30
自引率
0.00%
发文量
77
审稿时长
4-8 weeks
期刊介绍: BMC Genetics is an open access, peer-reviewed journal that considers articles on all aspects of inheritance and variation in individuals and among populations.
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