哺乳动物中有内含子滑动吗?

IF 3.4 Q1 Agricultural and Biological Sciences
Irina V Poverennaya, Nadezhda A Potapova, Sergey A Spirin
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引用次数: 4

摘要

背景:真核生物的蛋白质编码基因由外显子和内含子组成。外显子-内含子边界在物种之间是保守的,因此只有在相当长的进化距离上才能观察到它们的变化。内含子在短距离内移动是最罕见的变化类型之一,被称为“内含子滑动”,但这一事件的真实性已经争论了很长时间。搜索内含子滑动的主要思路是使用最准确的基因组注释和基因组序列,以及高质量的转录组数据。我们将其应用于哺乳动物中滑动内含子的搜索,以扩大对该群体中存在或不存在此类现象的认识。结果:在灵长类动物(人类、黑猩猩、恒河猴、食蟹猴、绿猴、狨猴)中未发现内含子滑动的明显证据。在EIF1AX人类和绵羊基因同源物之间只观察到一组高质量转录组支持的内含子滑动事件。此外,我们检查了先前在哺乳动物中观察到的内含子滑动事件列表,并表明它们很可能是基因组注释的产物,并且在随后的注释版本中没有显示出来,也没有转录组学数据支持。结论:我们假设内含子滑动确实是一个非常罕见的进化事件,如果它存在的话。每一例内含子滑动都需要大量的支持性数据进行检测和确认。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Is there any intron sliding in mammals?

Is there any intron sliding in mammals?

Is there any intron sliding in mammals?

Is there any intron sliding in mammals?

Background: Eukaryotic protein-coding genes consist of exons and introns. Exon-intron borders are conserved between species and thus their changes might be observed only on quite long evolutionary distances. One of the rarest types of change, in which intron relocates over a short distance, is called "intron sliding", but the reality of this event has been debated for a long time. The main idea of a search for intron sliding is to use the most accurate genome annotation and genome sequence, as well as high-quality transcriptome data. We applied them in a search for sliding introns in mammals in order to widen knowledge about the presence or absence of such phenomena in this group.

Results: We didn't find any significant evidence of intron sliding in the primate group (human, chimpanzee, rhesus macaque, crab-eating macaque, green monkey, marmoset). Only one possible intron sliding event supported by a set of high quality transcriptomes was observed between EIF1AX human and sheep gene orthologs. Also, we checked a list of previously observed intron sliding events in mammals and showed that most likely they are artifacts of genome annotations and are not shown in subsequent annotation versions as well as are not supported by transcriptomic data.

Conclusions: We assume that intron sliding is indeed a very rare evolutionary event if it exists at all. Every case of intron sliding needs a lot of supportive data for detection and confirmation.

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来源期刊
BMC Evolutionary Biology
BMC Evolutionary Biology 生物-进化生物学
CiteScore
5.80
自引率
0.00%
发文量
0
审稿时长
6 months
期刊介绍: BMC Evolutionary Biology is an open access, peer-reviewed journal that considers articles on all aspects of molecular and non-molecular evolution of all organisms, as well as phylogenetics and palaeontology.
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