大猩猩fosmid克隆HoxA基因簇的核苷酸测序。

Journal of Genomics Pub Date : 2020-08-29 eCollection Date: 2020-01-01 DOI:10.7150/jgen.50468

Takashi Kitano, Choong-Gon Kim, Naruya Saitou

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引用次数: 0

摘要

利用7个fosmid克隆对西部大猩猩(gorilla gorilla) HoxA聚类区进行测序，发现从HoxA1的5′非基因区到Evx1的3′非基因区，总平纹路径序列为214,185 bp。我们将该核苷酸序列与NCBI数据库中的大猩猩基因组序列进行比较，估计总核苷酸差异比例为0.0005-0.0007。这些估计值低于大猩猩的整体基因组多态性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Nucleotide sequencing of the HoxA gene cluster using Gorilla fosmid clones.

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Nucleotide sequencing of the HoxA gene cluster using Gorilla fosmid clones.

We sequenced the western gorilla (Gorilla gorilla) HoxA cluster region using seven fosmid clones, and found that the total tiling path sequence was 214,185 bp from the 5' non-genic region of HoxA1 to the 3' non-genic region of Evx1. We compared the nucleotide sequence with the gorilla genome sequence in the NCBI database, and the overall proportion of nucleotide difference was estimated to be 0.0005-0.0007. These estimates are lower than overall genomic polymorphism in gorillas.

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来源期刊

Journal of Genomics

自引率

0.00%

发文量

审稿时长

12 weeks

期刊介绍： Journal of Genomics publishes papers of high quality in all areas of gene, genetics, genomics, proteomics, metabolomics, DNA/RNA, computational biology, bioinformatics, and other relevant areas of research and application. Articles published by the journal are rigorously peer-reviewed. Types of articles include: Research paper, Short research communication, Review or mini-reviews, Commentary, Database, Software.