Kaiyue Chen, Nan Rong, Shujing Wang, Chunxiong Luo
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引用次数: 6
摘要
目前用于研究多环境(如n型)的多细胞菌株(如m型)的微流体方法需要大量的入口/出口(m*n),复杂的程序或昂贵的机器。本文提出了一种新的双层集成方法,通过PDMS通孔阵列将不同功能的PDMS微通道层组合在一个芯片上,从而改进了基于PDMS的微流控系统的设计。利用该方法,我们成功地将2 × m × n个入口/出口转换为m + n个入口/出口,并减少了在不同的多时间环境(即n型)下研究多细胞菌株(如m型)的设备加载处理时间成本(从m × n到m)。利用该装置,研究了当葡萄糖浓度从2%下降到一系列较低浓度时,细胞应激反应蛋白的动态行为。该装置还可广泛用于各种应力响应的高通量研究,多层集成制造方法的新概念可大大改善基于pdm的微流体系统的设计。
A novel two-layer-integrated microfluidic device for high-throughput yeast proteomic dynamics analysis at the single-cell level.
Current microfluidic methods for studying multicell strains (e.g., m-types) with multienvironments (e.g., n-types) require large numbers of inlets/outlets (m*n), a complicated procedure or expensive machinery. Here, we developed a novel two-layer-integrated method to combine different PDMS microchannel layers with different functions into one chip by a PDMS through-hole array, which improved the design of a PDMS-based microfluidic system. Using this method, we succeeded in converting 2 × m × n inlets/outlets into m + n inlets/outlets and reduced the time cost of loading processing (from m × n to m) of the device for studying multicell strains (e.g., m-types) in varied multitemporal environments (i.e., n-types). Using this device, the dynamic behavior of the cell-stress-response proteins was studied when the glucose concentration decreased from 2% to a series of lower concentrations. Our device could also be widely used in high-throughput studies of various stress responses, and the new concept of a multilayer-integrated fabrication method could greatly improve the design of PDMS-based microfluidic systems.