Kaihua Zhong, Shuxin Fan, Shujun Yao, Haibin Xu, Suping Bai
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引用次数: 1
摘要
本研究成功地从苍术根茎中分离得到了一种平均分子量为6.63 × 104 Da的水碱溶多糖(ALP)。GC分析表明ALP是葡聚糖的一种。在人骨肉瘤U-2 OS细胞中检测了ALP对e -选择素与唾液Lewis X (sLex)相互作用的影响。荧光显微镜下可见U-2 OS细胞表面有明显的sLex抗原表达。添加ALP(0.5、1和2 mg/mL)可显著抑制U-2 OS细胞对人脐静脉内皮细胞(HUVECs)的粘附、迁移和侵袭,这是通过降低U-2 OS细胞上sLex的表达实现的。流式细胞术TUNEL染色和Annexin V-FITC/PI双染色分析表明,ALP对U-2 OS细胞有诱导凋亡作用。综上所述,这些结果表明ALP通过抑制sLex / e -选择素的结合对骨肉瘤细胞具有抗转移活性,这表明ALP可能是一种有效的骨肉瘤干预药物。
A Atractylodes lancea polysaccharide inhibits metastasis of human osteosarcoma U-2 OS cells by blocking sialyl Lewis X (sLex )/E-selectin binding.
In this study, a new water and alkaline-soluble polysaccharide (ALP), with an average molecular weight of 6.63 × 104 Da, was successfully purified from the rhizomes of Atractylodes lancea. GC analysis demonstrated that ALP was a kind of glucan. The effect of the ALP on the interaction between E-selectin and sialyl Lewis X (sLex ) was examined in human osteosarcoma U-2 OS cells. It was obvious that the expression of sLex antigen on the surface of U-2 OS cells was visible under fluorescence microscopy. The addition of ALP (0.5, 1 and 2 mg/mL) resulted in a marked inhibition on the adhesion, migration and invasion of U-2 OS cells to human umbilical vein endothelial cells (HUVECs), which was achieved by the decreased sLex expression on U-2 OS cells. Additionally, the induction of apoptosis can be observed in U-2 OS cells following ALP treatment using TUNEL staining and Annexin V-FITC/PI double-staining analysis on flow cytometry. In conclusion, these results indicated that ALP exerted anti-metastatic activity towards osteosarcoma cells via inhibition of sLex /E-selectin binding, which suggested that ALP could be a potent agent for human osteosarcoma intervention.
期刊介绍:
Bridging physiology and cellular medicine, and molecular biology and molecular therapeutics, Journal of Cellular and Molecular Medicine publishes basic research that furthers our understanding of the cellular and molecular mechanisms of disease and translational studies that convert this knowledge into therapeutic approaches.