hpscs -心肌细胞低温保存与恢复的简单工作流程及培养基比较

Q2 Biochemistry, Genetics and Molecular Biology

Current Protocols in Stem Cell Biology Pub Date : 2020-09-21 DOI:10.1002/cpsc.125

Duncan C. Miller, Carolin Genehr, Narasimha S. Telugu, Silke Kurths, Sebastian Diecke

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引用次数: 4

摘要

近年来，人类造血干细胞-心肌细胞(hpscs - cms)的分化和功能应用研究取得了很大进展;然而，hscs - cms的冷冻保存和恢复仍然存在挑战，很少有报道详细描述了协议和一般工作流程。为了促进超低温保存和恢复hscs - cms的广泛应用，我们提供了详细的信息和一步一步的协议。该方法简单，使用常用试剂。它们由快速分离，使用标准设备冷冻保存和解冻后的温和恢复组成。我们讨论了协议的各种特性，以及它们在通用hPSC-CM区分和应用程序工作流环境中的使用。最后，我们比较了两种专有和两种常见的用于hscs - cms的冷冻保存培养基配方，尽管它们的价格和成分不同，但解冻后的回收率和细胞功能大致相似。©2019作者。基本方案1:解离和低温保存hpscs - cms基本方案2:解冻和低温冷冻的hpscs - cms恢复

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Simple Workflow and Comparison of Media for hPSC-Cardiomyocyte Cryopreservation and Recovery

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Simple Workflow and Comparison of Media for hPSC-Cardiomyocyte Cryopreservation and Recovery

Great progress has been made with protocols for the differentiation and functional application of hPSC-cardiomyocytes (hPSC-CMs) in recent years; however, the cryopreservation and recovery of hPSC-CMs still presents challenges and few reports describe in detail the protocols and general workflow. In order to facilitate cryopreservation and recovery of hPSC-CMs for a wide range of applications, we provide detailed information and step-by-step protocols. The protocols are simple and use common reagents. They are comprised of a fast dissociation, cryopreservation using standard equipment, and gentle recovery following thawing. We discuss various features of the protocols, as well as their utilization in the context of common hPSC-CM differentiation and application workflows. Finally, we compare two proprietary and two common in-house formulations of cryopreservation media used for hPSC-CMs, and despite differences in their price and composition find broadly similar recovery rates and cellular function after thawing. © 2019 The Authors.

Basic Protocol 1: Dissociation and cryopreservation of hPSC-CMs

Basic Protocol 2: Thawing and recovery of cryogenically frozen hPSC-CMs

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来源期刊

Current Protocols in Stem Cell Biology Biochemistry, Genetics and Molecular Biology-Cell Biology

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期刊介绍： Published in affiliation with the International Society for Stem Cell Research (ISSCR), Current Protocols in Stem Cell Biology (CPSC) covers the most fundamental protocols and methods in the rapidly growing field of stem cell biology. Updated monthly, CPSC will constantly evolve with thelatest developments and breakthroughs in the field. Drawing on the expertise of leading researchers from around the world, Current Protocols in Stem Cell Biology includes methods and insights that will enhance the progress of global research.