水泡性口炎病毒的繁殖、定量和储存。

Current Protocols in Microbiology Pub Date : 2020-09-01 DOI:10.1002/cpmc.110

Alaa A Abdelmageed, Maureen C Ferran

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引用次数: 10

摘要

水疱性口炎病毒(VSV)是横纹肌病毒科负义单链RNA病毒的典型成员。几十年来，这种病毒一直被用作强大的模型系统，目前正被用作疫苗平台和溶瘤剂。在这里，我们提出了传播、定量和存储VSV的方法。我们还审查了处理VSV的适当安全方案，VSV被美国疾病控制和预防中心列为生物安全2级病原体。©2020 Wiley期刊有限责任公司基本方案1:水疱性口炎病毒库存的产生、纯化和储存基本方案2:通过斑块测定法定量水疱性口炎病毒支持方案:Vero细胞的繁殖。

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The Propagation, Quantification, and Storage of Vesicular Stomatitis Virus.

Vesicular stomatitis virus (VSV) is the prototypical member of the Rhabdoviridae family of negative-sense single-stranded RNA viruses. This virus has been used as a powerful model system for decades and is currently being used as a vaccine platform and an oncolytic agent. Here, we present methods to propagate, quantitate, and store VSV. We also review the proper safety protocol for the handling of VSV, which is classified as a Biosafety Level 2 pathogen by the United States Centers for Disease Control and Prevention. © 2020 Wiley Periodicals LLC. Basic Protocol 1: Generation, purification, and storage of vesicular stomatitis virus stocks Basic Protocol 2: Quantification of vesicular stomatitis virus by plaque assay Support Protocol: Propagation of Vero cells.

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来源期刊

Current Protocols in Microbiology Immunology and Microbiology-Parasitology

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期刊介绍： Current Protocols in Microbiology provides detailed, step-by-step instructions for analyzing bacteria, animal and plant viruses, fungi, protozoans and other microbes. It offers updated coverage of emerging technologies and concepts, such as biofilms, quorum sensing and quantitative PCR, as well as proteomic and genomic methods. It is the first comprehensive source of high-quality microbiology protocols that reflects and incorporates the new mandates and capabilities of this robust and rapidly evolving discipline.