{"title":"cggbp1调节ctcf结合基序上的胞嘧啶甲基化抵抗随机性。","authors":"Manthan Patel, Divyesh Patel, Subhamoy Datta, Umashankar Singh","doi":"10.1186/s12863-020-00894-8","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>The human CGGBP1 binds to GC-rich regions and interspersed repeats, maintains homeostasis of stochastic cytosine methylation and determines DNA-binding of CTCF. Interdependence between regulation of cytosine methylation and CTCF occupancy by CGGBP1 remains unknown.</p><p><strong>Results: </strong>By analyzing methylated DNA-sequencing data obtained from CGGBP1-depleted cells, we report that some transcription factor-binding sites, including CTCF, resist stochastic changes in cytosine methylation. By analysing CTCF-binding sites we show that cytosine methylation changes at CTCF motifs caused by CGGBP1 depletion resist stochastic changes. These CTCF-binding sites are positioned at locations where the spread of cytosine methylation in cis depends on the levels of CGGBP1.</p><p><strong>Conclusion: </strong>Our findings suggest that CTCF occupancy and functions are determined by CGGBP1-regulated cytosine methylation patterns.</p>","PeriodicalId":9197,"journal":{"name":"BMC Genetics","volume":" ","pages":"84"},"PeriodicalIF":2.9000,"publicationDate":"2020-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12863-020-00894-8","citationCount":"5","resultStr":"{\"title\":\"CGGBP1-regulated cytosine methylation at CTCF-binding motifs resists stochasticity.\",\"authors\":\"Manthan Patel, Divyesh Patel, Subhamoy Datta, Umashankar Singh\",\"doi\":\"10.1186/s12863-020-00894-8\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>The human CGGBP1 binds to GC-rich regions and interspersed repeats, maintains homeostasis of stochastic cytosine methylation and determines DNA-binding of CTCF. Interdependence between regulation of cytosine methylation and CTCF occupancy by CGGBP1 remains unknown.</p><p><strong>Results: </strong>By analyzing methylated DNA-sequencing data obtained from CGGBP1-depleted cells, we report that some transcription factor-binding sites, including CTCF, resist stochastic changes in cytosine methylation. By analysing CTCF-binding sites we show that cytosine methylation changes at CTCF motifs caused by CGGBP1 depletion resist stochastic changes. These CTCF-binding sites are positioned at locations where the spread of cytosine methylation in cis depends on the levels of CGGBP1.</p><p><strong>Conclusion: </strong>Our findings suggest that CTCF occupancy and functions are determined by CGGBP1-regulated cytosine methylation patterns.</p>\",\"PeriodicalId\":9197,\"journal\":{\"name\":\"BMC Genetics\",\"volume\":\" \",\"pages\":\"84\"},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2020-07-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1186/s12863-020-00894-8\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"BMC Genetics\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1186/s12863-020-00894-8\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"Biochemistry, Genetics and Molecular Biology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"BMC Genetics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1186/s12863-020-00894-8","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
CGGBP1-regulated cytosine methylation at CTCF-binding motifs resists stochasticity.
Background: The human CGGBP1 binds to GC-rich regions and interspersed repeats, maintains homeostasis of stochastic cytosine methylation and determines DNA-binding of CTCF. Interdependence between regulation of cytosine methylation and CTCF occupancy by CGGBP1 remains unknown.
Results: By analyzing methylated DNA-sequencing data obtained from CGGBP1-depleted cells, we report that some transcription factor-binding sites, including CTCF, resist stochastic changes in cytosine methylation. By analysing CTCF-binding sites we show that cytosine methylation changes at CTCF motifs caused by CGGBP1 depletion resist stochastic changes. These CTCF-binding sites are positioned at locations where the spread of cytosine methylation in cis depends on the levels of CGGBP1.
Conclusion: Our findings suggest that CTCF occupancy and functions are determined by CGGBP1-regulated cytosine methylation patterns.
期刊介绍：
BMC Genetics is an open access, peer-reviewed journal that considers articles on all aspects of inheritance and variation in individuals and among populations.
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