GHK-Cu肽和58S生物玻璃表面修饰增强胶原/壳聚糖包被聚(ε-己内酯)支架的生物学性能。

IF 4.4 3区 医学 Q2 ENGINEERING, BIOMEDICAL
Progress in Biomaterials Pub Date : 2020-06-01 Epub Date: 2020-04-04 DOI:10.1007/s40204-020-00129-0
Amir Mahdi Molavi, Alireza Sadeghi-Avalshahr, Samira Nokhasteh, Hojjat Naderi-Meshkin
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引用次数: 8

摘要

生物活性玻璃和多肽在促进伤口愈合和皮肤修复方面显示出有希望的结果。本研究探讨了58S生物活性玻璃或GHK-Cu肽对胶原/壳聚糖包被静电纺丝聚(ε-己内酯)支架的表面改性效果。为了将生物活性玻璃涂在支架上,我们制备了三种不同浓度的硅化生物活性玻璃粉悬浮液,并用悬浮液对支架进行移液。同样,将1-mM的肽溶液(毫升- q)移液于支架表面即可制备ghk - cu包被支架。ATR-FTIR光谱分析表明,生物活性玻璃和GHK-Cu成功修饰了胶原/壳聚糖包被的静电纺丝聚(ε-己内酯)支架。进行了显微结构观察和体外细胞粘附、细胞活力和抗菌实验等研究。所有样品均表现出良好的细胞附着。与聚(ε-己内酯)/胶原/壳聚糖相比,GHK-Cu和生物活性玻璃包被(浓度分别为0.01和0.1)支架的细胞增殖在第3天和第7天分别显著增加。聚(ε-己内酯)/胶原/壳聚糖-未包被支架和包被GHK-Cu和生物活性玻璃的支架均显示出良好的抗菌性能,但包被GHK-Cu的支架的抗菌活性更强。这些结果表明,GHK-Cu和生物活性玻璃可以改善胶原/壳聚糖包被合成聚合物的生物学性能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Enhanced biological properties of collagen/chitosan-coated poly(ε-caprolactone) scaffold by surface modification with GHK-Cu peptide and 58S bioglass.

Bioactive glasses and peptides have shown promising results in improving wound healing and skin repair. The present study explores the effectiveness of surface modification of collagen/chitosan-coated electrospun poly(ε-caprolactone) scaffold with 58S bioactive glass or GHK-Cu peptide. To coat scaffolds with the bioactive glass, we prepared suspensions of silanized bioactive glass powder with three different concentrations and the scaffolds were pipetted with suspensions. Similarly, GHK-Cu-coated scaffolds were prepared by pipetting adequate amount of 1-mM solution of peptide (in milli-Q) on the surface of scaffolds. ATR-FTIR spectroscopy indicated the successful modification of collagen/chitosan-coated electrospun poly(ε-caprolactone) scaffold with bioactive glass and GHK-Cu. Microstructural investigations and in vitro studies such as cell adhesion, cell viability and antibacterial assay were performed. All samples demonstrated desirable cell attachment. Compared to poly(ε-caprolactone)/collagen/chitosan, the cell proliferation of GHK-Cu and bioactive glass-coated (concentrations of 0.01 and 0.1) scaffolds increased significantly at days 3 and 7, respectively. Poly(ε-caprolactone)/collagen/chitosan-uncoated scaffold and scaffolds coated with GHK-Cu and bioactive glass revealed desirable antibacterial properties but the antibacterial activity of GHK-Cu-coated sample turned out to be superior. These findings indicated that biological properties of collagen/chitosan-coated synthetic polymer could be improved by GHK-Cu and bioactive glass.

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来源期刊
Progress in Biomaterials
Progress in Biomaterials MATERIALS SCIENCE, BIOMATERIALS-
CiteScore
9.60
自引率
4.10%
发文量
35
期刊介绍: Progress in Biomaterials is a multidisciplinary, English-language publication of original contributions and reviews concerning studies of the preparation, performance and evaluation of biomaterials; the chemical, physical, biological and mechanical behavior of materials both in vitro and in vivo in areas such as tissue engineering and regenerative medicine, drug delivery and implants where biomaterials play a significant role. Including all areas of: design; preparation; performance and evaluation of nano- and biomaterials in tissue engineering; drug delivery systems; regenerative medicine; implantable medical devices; interaction of cells/stem cells on biomaterials and related applications.
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