真核细胞DNA和RNA成像揭示基因表达的时空动态。

IF 12.1 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Annual review of biochemistry Pub Date : 2020-06-20 Epub Date: 2020-03-16 DOI:10.1146/annurev-biochem-011520-104955
Hanae Sato, Sulagna Das, Robert H Singer, Maria Vera
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引用次数: 32

摘要

本文综述了活细胞中DNA和单RNA分子的成像,以定义真核生物的功能组织和动态过程。讨论了实时可视化单个DNA位点和rna技术的最新进展。单分子荧光显微镜提供了空间和时间分辨率来揭示调节基本细胞功能的机制。本文综述了多色荧光显微镜对核结构、转录、转录后RNA加工和RNA定位调控的新见解。展望未来使用的实时成像技术和克服其目前的局限性提供。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Imaging of DNA and RNA in Living Eukaryotic Cells to Reveal Spatiotemporal Dynamics of Gene Expression.

This review focuses on imaging DNA and single RNA molecules in living cells to define eukaryotic functional organization and dynamic processes. The latest advances in technologies to visualize individual DNA loci and RNAs in real time are discussed. Single-molecule fluorescence microscopy provides the spatial and temporal resolution to reveal mechanisms regulating fundamental cell functions. Novel insights into the regulation of nuclear architecture, transcription, posttranscriptional RNA processing, and RNA localization provided by multicolor fluorescence microscopy are reviewed. A perspective on the future use of live imaging technologies and overcoming their current limitations is provided.

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来源期刊
Annual review of biochemistry
Annual review of biochemistry 生物-生化与分子生物学
CiteScore
33.90
自引率
0.00%
发文量
31
期刊介绍: The Annual Review of Biochemistry, in publication since 1932, sets the standard for review articles in biological chemistry and molecular biology. Since its inception, these volumes have served as an indispensable resource for both the practicing biochemist and students of biochemistry.
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