非cpg甲基化偏差亚硫酸盐PCR对低或未甲基化线粒体DNA:对该领域的建议。

IF 4.8 Q1 GENETICS & HEREDITY
Environmental Epigenetics Pub Date : 2020-02-04 eCollection Date: 2020-01-01 DOI:10.1093/eep/dvaa001
Margaret J Morris, Luke B Hesson, Neil A Youngson
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引用次数: 7

摘要

线粒体DNA (mtDNA)是一个16kb的环状基因组,存在于线粒体的多个拷贝中。mtDNA编码有助于线粒体结构和功能的基因。一个长期存在的问题是,mtDNA是否受到与核基因组相似的表观遗传调控。最近发表的数据表明,与CpG甲基化是常态的核基因组不同,mtDNA主要在非CpG胞嘧啶上甲基化。这为今后的研究提出了重要的方法学考虑。特别是,现有的亚硫酸盐PCR技术可能不适合,因为引物偏向于扩增未甲基化的mtDNA。在这里,我们描述了这可能导致之前的研究低估了mtDNA甲基化水平,并重申了准确评估mtDNA甲基化的方法策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Non-CpG methylation biases bisulphite PCR towards low or unmethylated mitochondrial DNA: recommendations for the field.

Non-CpG methylation biases bisulphite PCR towards low or unmethylated mitochondrial DNA: recommendations for the field.

Mitochondrial DNA (mtDNA) is a circular genome of 16 kb that is present in multiple copies in mitochondria. mtDNA codes for genes that contribute to mitochondrial structure and function. A long-standing question has asked whether mtDNA is epigenetically regulated similarly to the nuclear genome. Recently published data suggest that unlike the nuclear genome where CpG methylation is the norm, mtDNA is methylated predominantly at non-CpG cytosines. This raises important methodological considerations for future investigations. In particular, existing bisulphite PCR techniques may be unsuitable due to primers being biased towards amplification from unmethylated mtDNA. Here, we describe how this may have led to previous studies underestimating the level of mtDNA methylation and reiterate methodological strategies for its accurate assessment.

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来源期刊
Environmental Epigenetics
Environmental Epigenetics GENETICS & HEREDITY-
CiteScore
6.50
自引率
5.30%
发文量
0
审稿时长
17 weeks
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