肾细胞癌原发肿瘤组织中的微生物组可能与静脉肿瘤血栓的PD-L1表达有关。

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Accounts of Chemical Research Pub Date : 2020-02-18 eCollection Date: 2020-01-01 DOI:10.1155/2020/9068068
Michael A Liss, Yidong Chen, Ronald Rodriguez, Deepak Pruthi, Teresa Johnson-Pais, Hanzhang Wang, Ahmed Mansour, James R White, Dharam Kaushik
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引用次数: 14

摘要

目的:在透明细胞肾细胞癌(cc-RCC)伴肿瘤血栓(TT)中进行微生物组评估和PD-L1表达谱的概念验证。方法:6例经IRB批准的患者行根治性肾切除术(RN)合并静脉肿瘤取栓术(VTT)。我们从正常邻近组织、肿瘤组织和血栓组织中采集新鲜组织标本。我们利用RNA测序获得PD-L1表达谱并进行微生物组分析。采用SPSS v25进行学生t检验、卡方检验和spearman秩相关分析。结果:我们注意到肿瘤血栓大多缺乏多种微生物群。检测到很大比例的表皮葡萄球菌,但不知道这是手术或手术后的污染物;然而,它在血栓中比在其他组织中更明显。与血栓或正常邻近组织相比,原发肿瘤中微生物组多样性最丰富。PD-L1在肿瘤血栓与正常背景组织中的差异表达被检测到,6名受试者中有3名阈值超过2倍。这三个相似的受试者有外来微生物群,是口腔微生物群的典型居民。结论:肾肿瘤具有比正常邻近组织更多样化的微生物组。透明细胞肾癌伴肿瘤血栓患者口腔微生物组谱的鉴定为血栓对PD-L1抑制的反应提供了一个潜在的生物标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Microbiome within Primary Tumor Tissue from Renal Cell Carcinoma May Be Associated with PD-L1 Expression of the Venous Tumor Thrombus.

Microbiome within Primary Tumor Tissue from Renal Cell Carcinoma May Be Associated with PD-L1 Expression of the Venous Tumor Thrombus.

Microbiome within Primary Tumor Tissue from Renal Cell Carcinoma May Be Associated with PD-L1 Expression of the Venous Tumor Thrombus.

Microbiome within Primary Tumor Tissue from Renal Cell Carcinoma May Be Associated with PD-L1 Expression of the Venous Tumor Thrombus.

Objective: To perform a proof of concept microbiome evaluation and PD-L1 expression profiling in clear-cell renal cell carcinoma (cc-RCC) with associated tumor thrombus (TT).

Methods: After IRB approval, six patients underwent radical nephrectomy (RN) with venous tumor thrombectomy (VTT). We collected fresh tissue specimens from normal adjacent, tumor, and thrombus tissues. We utilized RNA sequencing to obtain PD-L1 expression profiles and perform microbiome analysis. Statistical assessment was performed using Student's t-test, chi-square, and spearman rank correlations using SPSS v25.

Results: We noted the tumor thrombus to be mostly devoid of diverse microbiota. A large proportion of Staphylococcus epidermidus was detected and unknown if this is a surgical or postsurgical contaminant; however, it was noted more in the thrombus than other tissues. Microbiome diversity profiles were most abundant in the primary tumor compared to the thrombus or normal adjacent tissue. Differential expression of PD-L1 was examined in the tumor thrombus to the normal background tissue and noted three of the six subjects had a threshold above 2-fold. These three similar subjects had foreign microbiota that are typical residents of the oral microbiome.

Conclusion: Renal tumors have more diverse microbiomes than normal adjacent tissue. Identification of resident oral microbiome profiles in clear-cell renal cancer with tumor thrombus provides a potential biomarker for thrombus response to PD-L1 inhibition.

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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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