Y Shaoqing, C Yinjian, Y Zhiqiang, Z Ruxin, C Na, G Rongming
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引用次数: 4
摘要
背景:CD4+CD25+调节性T细胞(CD4+CD25+ TRegs)参与过敏性疾病已有报道。然而，CD4+CD25+ treg是否参与变应性鼻炎(AR)尚不清楚。方法:采用流式细胞术检测20例AR患者新鲜全血和16例健康献血者CD4+CD25+和CD4+CD25hi Treg细胞的频率。此外，采用酶联免疫吸附法测定血清总IgE (IU/mL)水平。结果:AR患者CD4+CD25+ Treg细胞(2.80±1.36%比3.94±0.97%，P < 0.01)和CD4+CD25hi Treg细胞(1.53±0.62%比2.00±0.52%，P < 0.05)低于对照组。CD4+CD25+和CD4+CD25hi treg数量与总免疫球蛋白E水平呈负相关(r = -0.79, P < 0.01, r = -0.61, P < 0.01)。结论:调节性T细胞缺陷可能在AR的发生发展中起一定作用。
The levels of CD4+CD25+ regulatory T cells in patients with allergic rhinitis.
Background: The involvement of CD4+CD25+ regulatory T cells (CD4+CD25+ TRegs) in allergic diseases was reported previously. However, it remains unclear whether CD4+CD25+ TRegs are involved in allergic rhinitis (AR).
Methods: Fresh whole blood from 20 patients with AR and 16 healthy donors was used to investigate the frequency of CD4+CD25+ and CD4+CD25hi Treg cells using flow cytometry. In addition, serum total IgE (IU/mL) levels were determined using enzyme-linked immunosorbent assays.
Results: Patients with AR had fewer CD4+CD25+ Treg cells (2.80 ± 1.36% vs. 3.94 ± 0.97%, P < 0.01) and CD4+CD25hi TRegs (1.53 ± 0·62% vs. 2.00 ± 0.52%, P < 0.05) than control subjects. The number of CD4+CD25+ and CD4+CD25hi TRegs was correlated negatively with total immunoglobulin E levels (r = -0.79, P < 0.01 and r = -0.61, P < 0.01, respectively).
Conclusion: Deficient regulatory T cells might play a role in the development of AR.
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