舌螺虫α- n -乙酰半乳糖胺酶转化红细胞的研究。

Q2 Biochemistry, Genetics and Molecular Biology

Enzyme Research Pub Date : 2019-05-02 eCollection Date: 2019-01-01 DOI:10.1155/2019/6972835

Thomas J Malinski, Harkewal Singh

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引用次数: 2

摘要

舌螺虫是一种自由生活的非致病性生物。像许多其他细菌一样，S. linguale产生与细胞相关的α- n -乙酰半乳糖胺酶。进行这项工作是为了阐明这项活动的性质。重组酶的产生、纯化和生化特性检测。纯化后的酶在溶液中为同二聚体，活性约50 kDa。在pH为7时催化α- n -乙酰半乳糖胺水解。计算KM为1.1 mM, kcat为173 s-1。所描述的酶属于GH109家族。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Enzymatic Conversion of RBCs by α-N-Acetylgalactosaminidase from Spirosoma linguale.

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Enzymatic Conversion of RBCs by α-N-Acetylgalactosaminidase from Spirosoma linguale.

Spirosoma linguale is a free-living nonpathogenic organism. Like many other bacteria, S. linguale produces a cell-associated α-N-acetylgalactosaminidase. This work was undertaken to elucidate the nature of this activity. The recombinant enzyme was produced, purified, and examined for biochemical attributes. The purified enzyme was ~50 kDa active as a homodimer in solution. It catalyzed hydrolysis of α-N-acetylgalactosamine at pH 7. Calculated K_M was 1.1 mM with k_cat of 173 s^-1. The described enzyme belongs to the GH109 family.

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来源期刊

Enzyme Research Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

4.60

自引率

0.00%

发文量