CD28和CTLA-4分子在急性髓性白血病患者中的异常表达及其与造血干细胞移植后移植物抗宿主病发展的可能关联

IF 0.3 Q4 TRANSPLANTATION
International Journal of Organ Transplantation Medicine Pub Date : 2019-01-01 Epub Date: 2019-05-01
M Ramzi, M Iravani Saadi, R Yaghobi, N Arandi
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引用次数: 0

摘要

背景:共刺激分子表达失调是急性髓性白血病(AML)等血液系统恶性肿瘤的免疫逃逸机制之一。目的:评价62例成人新生AML患者CD28和CTLA-4分子的表达及其与造血干细胞移植后急性移植物抗宿主病(GVHD)发生的相关性。方法:采用SYBR Green实时荧光定量PCR法检测CD28和CTLA-4在患者组、对照组以及不同风险组(高、中、高危)、M3与非M3、GVHD与非GVHD患者中的相对表达。结果:AML患者CD28(7.9倍)和CTLA-4(5.7倍)mRNA表达量较健康对照组显著升高(p分别=0.006和0.02)。高危组CD28和CTLA-4的平均表达虽然较低危、中危组升高,但差异无统计学意义。此外,M3患者中CTLA-4的平均表达明显高于非M3患者,而CD28的平均表达明显高于非M3患者(p结论:CD28和CTLA-4分子在AML患者外周血白细胞中异常表达,可能与造血干细胞移植后aGVHD的发生有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Dysregulated Expression of CD28 and CTLA-4 Molecules in Patients with Acute Myeloid Leukemia and Possible Association with Development of Graft <i>versus</i> Host Disease after Hematopoietic Stem Cell Transplantation.

Dysregulated Expression of CD28 and CTLA-4 Molecules in Patients with Acute Myeloid Leukemia and Possible Association with Development of Graft versus Host Disease after Hematopoietic Stem Cell Transplantation.

Background: Dysregulated expression of co-stimulatory molecules is one of the immune escape mechanisms employed in hematologic malignancies like acute myeloid leukemia (AML).

Objectives: To evaluate the expression of the CD28 and CTLA-4 molecules in 62 adults with de novo AML and its correlation with the development of acute graft vs host disease (GVHD) after hematopoietic stem-cell transplantation.

Methods: The relative expression of CD28 and CTLA-4 was measured by quantitative SYBR Green real-time PCR method in a group of patients and controls as well as different risk groups (high, intermediate and favorite risk), M3 vs non-M3 and GVHD vs non-GVHD patients.

Results: The mRNA expression of CD28 (7.9-fold) and CTLA-4 (5.7-fold) was significantly increased in AML patients compared with healthy controls (p=0.006 and 0.02, respectively). Although the mean expression of both CD28 and CTLA-4 was increased in high-risk group compared with low-risk and intermediate-risk groups, the difference was not statistically significant. Also, the mean expression of the CTLA-4, but not CD28, was significantly higher in M3 patients compared with non-M3 ones (p<0.001). The expression of CD28 was upregulated in GVHD patients, while the expression of CTLA-4 was slightly lower in GVHD patients compared with non-GVHD patients, though the difference was not statistically significant. There was no significant correlation between the expression of CD28 and CTLA-4 and laboratory parameters like white blood cells and platelets counts, and hemoglobin and lactate dehydrogenase level in AML patients.

Conclusions: CD28 and CTLA-4 molecules are aberrantly expressed in peripheral blood leukocytes of AML patients and might contribute to the development of aGVHD after hematopoietic stem cell transplantation.

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来源期刊
CiteScore
1.60
自引率
0.00%
发文量
0
审稿时长
12 weeks
期刊介绍: The International Journal of Organ Transplantation Medicine (IJOTM) is a quarterly peer-reviewed English-language journal that publishes high-quality basic sciences and clinical research on transplantation. The scope of the journal includes organ and tissue donation, procurement and preservation; surgical techniques, innovations, and novelties in all aspects of transplantation; genomics and immunobiology; immunosuppressive drugs and pharmacology relevant to transplantation; graft survival and prevention of graft dysfunction and failure; clinical trials and population analyses in the field of transplantation; transplant complications; cell and tissue transplantation; infection; post-transplant malignancies; sociological and ethical issues and xenotransplantation.
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