乙肝病毒感染肝移植受者有或无排斥反应时IRF1基因表达与肝酶的关系

IF 0.3 Q4 TRANSPLANTATION
International Journal of Organ Transplantation Medicine Pub Date : 2018-01-01 Epub Date: 2018-05-01
S H Nabavizadeh, S Janfeshan, M H Karimi, A Eidi, R Yaghobi, A Afshari, B Geramizadeh, S A Malek-Hosseini, F Kafilzadeh
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引用次数: 0

摘要

背景:慢性乙型肝炎病毒(HBV)感染肝移植受者肝功能指标和抗病毒免疫调节标志物均可改善移植预后,从而改善移植后管理,增加监测的可能性。目的:探讨有或无排斥反应的乙肝病毒感染肝移植受者干扰素调节因子1 (IRF1) mRNA水平与肝酶的关系。方法:将46例接受肝移植手术的慢性乙肝病毒感染者分为“有排斥反应”组和“无排斥反应”组。在移植后第1、4和7天采集每位患者的血样。采用SYBER GREEN实时荧光定量PCR检测IRF1在肝受体中的表达水平。同时测量所有患者的肝酶活性。结果:与无排斥反应的患者相比,有排斥反应的患者IRF1的表达在所有3天的随访中均上调。两组患者随访3次血清ALT、AST水平均高于正常水平。移植后7天,有排斥反应和无排斥反应的患者IRF1基因表达水平和血清ALT水平均有显著差异。结论:排斥反应患者IRF1表达及血清ALT水平明显高于无排斥反应患者。IRF1作为一种炎症因子,也可能在移植物排斥患者中加强对移植肝脏炎症通路的诱导,促进肝脏炎症和损伤,导致肝酶升高。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Association between <i>IRF1</i> Gene Expression and Liver Enzymes in HBV-infected Liver Transplant Recipients with and without Experience of Rejection.

Association between <i>IRF1</i> Gene Expression and Liver Enzymes in HBV-infected Liver Transplant Recipients with and without Experience of Rejection.

Association between IRF1 Gene Expression and Liver Enzymes in HBV-infected Liver Transplant Recipients with and without Experience of Rejection.

Background: Liver function indices and anti-viral immune regulatory markers can both improve graft outcomes, which lead to better post-transplantation management and increase the possibility of surveillance in liver transplant recipients with chronic hepatitis B virus (HBV) infection.

Objective: To determine the association between the interferon regulatory factor 1 (IRF1) mRNA levels and liver enzymes in HBV-infected liver transplant recipients with and without experience of rejection.

Methods: A total of 46 chronic HBV-infected patients who had undergone liver transplant surgery was divided into 2 groups of recipients "with rejection" and "without rejection.". Blood samples were collected form each patient on days 1, 4, and 7 post-transplantation. A SYBER GREEN real-time PCR was used to evaluate the expression level of IRF1 in liver recipients. Liver enzyme activities were also measured in all patients.

Results: The expression of IRF1 in the patients with rejection was up-regulated at all 3 follow-up days compared with those without rejection. The serum levels of ALT and AST were more than normal levels at 3 follow-up times in both study groups. Significant differences were found in IRF1 gene expression levels and also serum ALT levels between those with and without rejection after 7 days post-transplantation.

Conclusion: The IRF1 expression and serum ALT levels were increased significantly in patient with rejection compared to those without rejection. IRF1, an inflammatory factor, may also intensify induction of inflammatory pathways in engrafted liver and promote liver inflammation and injuries leading to liver enzymes elevation in patients with graft rejection.

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来源期刊
CiteScore
1.60
自引率
0.00%
发文量
0
审稿时长
12 weeks
期刊介绍: The International Journal of Organ Transplantation Medicine (IJOTM) is a quarterly peer-reviewed English-language journal that publishes high-quality basic sciences and clinical research on transplantation. The scope of the journal includes organ and tissue donation, procurement and preservation; surgical techniques, innovations, and novelties in all aspects of transplantation; genomics and immunobiology; immunosuppressive drugs and pharmacology relevant to transplantation; graft survival and prevention of graft dysfunction and failure; clinical trials and population analyses in the field of transplantation; transplant complications; cell and tissue transplantation; infection; post-transplant malignancies; sociological and ethical issues and xenotransplantation.
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