Lipid peroxidation, thiol groups, and total antioxidant capacity in mothers in natural vaginal delivery with and without Entonox: A cohort study.

Background and objectives: Considering that very few studies have been conducted on the effect of Entonox on oxidative stress markers, this study was conducted to compare these markers in natural vaginal delivery (NVD) with and without Entonox.

Materials and methods: The present cohort study was conducted on 120 women in maternity wards divided into two groups, including an NVD group with and an NVD group without Entonox. After obtaining the mothers' written consent, 5 cc of venous blood was taken at the end of their second stage of labor for lipid peroxidation, thiol groups, and total antioxidant capacity tests. The data obtained were analyzed using descriptive (mean) and inferential (t-test) statistics.

Results: Lipid peroxide was 6.267 ± 5.39 in NVD without Entonox and 5.12 ± 3.89 in NVD with Entonox, suggesting the lack of a statistically significant difference (p = 0.191). Thiol marker was 0.34 ± 0.26 in NVD without and 0.26 ± 0.24 in NVD with Entonox, suggesting the lack of a statistically significant difference (p = 0.09).The FRAP results were 0.936 ± 0.696 in NVD without and 1.21 ± 0.89 in NVD with Entonox, suggesting the lack of a statistically significant difference (p = 0.06).

Discussion: Entonox can be safely used in NVD without increasing the risk of oxidative stress.