利用核酸酶S1进行基因组DNA位点选择性切割的人工限制性DNA切割器

Q4 Chemistry

Current Protocols in Nucleic Acid Chemistry Pub Date : 2019-02-05 DOI:10.1002/cpnc.72

Arivazhagan Rajendran, Narumi Shigi, Jun Sumaoka, Makoto Komiyama

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Artificial Restriction DNA Cutter Using Nuclease S1 for Site-Selective Scission of Genomic DNA

By combining a pair of pseudo-complementary peptide nucleic acids (pcPNAs) with S1 nuclease, a novel tool to cut DNA at a predetermined site can be obtained. Complementary pcPNAs invade the DNA duplex and base pair to each strand of a target site, creating single-stranded regions that are cleaved by S1 nuclease. The scission site can be freely modulated by the design of pcPNAs. This method can be used to cleave a single site in the human genome. This protocol presents experimental details for site-selective scission using this versatile new tool. © 2019 by John Wiley & Sons, Inc.

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Current Protocols in Nucleic Acid Chemistry Chemistry-Organic Chemistry

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期刊介绍： Published in association with International Society for Nucleosides, Nucleotides & Nucleic Acids (IS3NA) , Current Protocols in Nucleic Acid Chemistry is equally valuable for biotech, pharmaceutical, and academic labs. It is the resource for designing and running successful research projects in the rapidly growing and changing field of nucleic acid, nucleotide, and nucleoside research.