刚果锥虫:体外培养与转染

Chris Kay, Lori Peacock, Wendy Gibson
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引用次数: 5

摘要

刚果锥虫与间日疟原虫和布氏锥虫一起引起非洲动物锥虫病,即纳加纳病,这是撒哈拉以南非洲地区由吸血采采蝇传播的一种牲畜疾病。这些寄生原生生物在两个宿主之间循环:哺乳动物和采采蝇。每个寄主提供给锥虫的环境明显不同,因此在哺乳动物中发现的代谢阶段不同于昆虫的阶段。对于新的诊断和治疗方法的研究,使用哺乳动物生命周期阶段的血流形式是合适的。昆虫阶段,如原环虫,对研究分化是有用的,也可以作为一个方便的来源,容易培养,非传染性的有机体。在这里,我们介绍了目前在我们的实验室中使用的方案,用于体外培养不同生命周期阶段的刚果T. -原环体,粘原体和血流形式-以及使生物体能够进行基因改造的转染方法。©2019 by John Wiley &儿子,Inc。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Trypanosoma congolense: In Vitro Culture and Transfection

Trypanosoma congolense, together with T. vivax and T. brucei, causes African animal trypanosomiasis (AAT), or nagana, a livestock disease carried by bloodsucking tsetse flies in sub-Saharan Africa. These parasitic protists cycle between two hosts: mammal and tsetse fly. The environment offered by each host to the trypanosome is markedly different, and hence the metabolism of stages found in the mammal differs from that of insect stages. For research on new diagnostics and therapeutics, it is appropriate to use the mammalian life cycle stage, bloodstream forms. Insect stages such as procyclics are useful for studying differentiation and also serve as a convenient source of easily cultured, non-infective organisms. Here, we present protocols in current use in our laboratory for the in vitro culture of different life cycle stages of T. congolense—procyclics, epimastigotes, and bloodstream forms—together with methods for transfection enabling the organism to be genetically modified. © 2019 by John Wiley & Sons, Inc.

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来源期刊
Current Protocols in Microbiology
Current Protocols in Microbiology Immunology and Microbiology-Parasitology
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期刊介绍: Current Protocols in Microbiology provides detailed, step-by-step instructions for analyzing bacteria, animal and plant viruses, fungi, protozoans and other microbes. It offers updated coverage of emerging technologies and concepts, such as biofilms, quorum sensing and quantitative PCR, as well as proteomic and genomic methods. It is the first comprehensive source of high-quality microbiology protocols that reflects and incorporates the new mandates and capabilities of this robust and rapidly evolving discipline.
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