多色流式细胞术分析造血干细胞和祖细胞的细胞周期

Q1 Health Professions

Current Protocols in Cytometry Pub Date : 2018-10-18 DOI:10.1002/cpcy.50

Amy Galvin, Meredith Weglarz, Kat Folz-Donahue, Maris Handley, Misa Baum, Michael Mazzola, Hannah Litwa, David T. Scadden, Lev Silberstein

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引用次数: 6

摘要

维持造血干细胞(HSC)的静止是自我更新和分化为成熟谱系的关键。因此，可靠地检测异常造血干细胞循环的能力对于研究造血干细胞功能异常的实验至关重要。在罕见的细胞子集中可重复地评估细胞周期状态的能力需要在细胞制备和样品处理过程中仔细优化多个参数。在这里，我们描述了一种方法，其中数据采集参数和用于长期HSC染色的荧光染料组合是专门为DAPI和Ki-67抗体同时使用而设计的。©2018 by John Wiley &儿子,Inc。

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Cell Cycle Analysis of Hematopoietic Stem and Progenitor Cells by Multicolor Flow Cytometry

Maintenance of hematopoietic stem cell (HSC) quiescence is critical for self-renewal and differentiation into mature lineages. Therefore, the ability to reliably detect abnormal HSC cycling is essential for experiments that seek to investigate abnormalities of HSC function. The ability to reproducibly evaluate cell cycle status in a rare cell subset requires careful optimization of multiple parameters during cell preparation and sample processing. Here, we describe a method where data acquisition parameters and fluorochrome combination for long-term HSC staining have been specifically designed for concurrent use with DAPI and Ki-67 antibodies. © 2018 by John Wiley & Sons, Inc.

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来源期刊

Current Protocols in Cytometry Health Professions-Medical Laboratory Technology

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期刊介绍： Published in affiliation with the International Society for Advancement of Cytometry, Current Protocols in Cytometry is a "best practices" collection that distills and organizes the absolute latest techniques from the top cytometry labs and specialists worldwide. It is the most complete set of peer-reviewed protocols for flow and image cytometry available.