皮肤t细胞淋巴瘤中缺乏实时检测皮肤病毒DNA的PCR方法。

IF 2 Q3 Medicine

Giornale Italiano Di Dermatologia E Venereologia Pub Date : 2020-12-01 Epub Date: 2018-10-29 DOI:10.23736/S0392-0488.18.06161-8

Massimiliano Bergallo, Valentina DaprÀ, Paolo Fava, Renata Ponti, Cristina Calvi, Maria T Fierro, Pietro Quaglino, Ilaria Galliano, Paola Montanari

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引用次数: 6

摘要

背景:一种新的人类原细小病毒被发现，命名为Cutavirus。我们使用real - time TaqMan®(Thermo Fisher Scientific, Waltham, MA, USA) PCR检测皮肤t细胞淋巴瘤样本中cutlavirus的存在。方法:在7500 ABI仪器上使用TaqMan®Real time PCR对55份CTCL样本进行分析。所有这些都显示了内部控制的放大。结果:检测到相应的冠状病毒DNA存在。未在任何皮肤标本中检测到CuV DNA序列。结论:刀具病毒在皮肤癌中的作用有待进一步研究。

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Lack of detection of Cutavirus DNA using PCR real time in cutaneous T-cell lymphomas.

Background: A novel human protoparvovirus named Cutavirus has been discovered. We investigated the presence of Cutavirus in a sample of Cutaneous T-cell lymphomas by using PCR real time TaqMan® (Thermo Fisher Scientific, Waltham, MA, USA).

Methods: In total, 55 CTCL samples were analyzed using a TaqMan® Real time PCR on a 7500 ABI instrument. All of these shown internal control amplification.

Results: The presence of Cutavirus DNA corresponding was examined. CuV DNA sequences were not detected in any skin specimen.

Conclusions: The role of Cutaviruses in cutaneous cancers remains to be investigated.

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来源期刊

Giornale Italiano Di Dermatologia E Venereologia DERMATOLOGY-

CiteScore

1.90

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The journal Giornale Italiano di Dermatologia e Venereologia publishes scientific papers on dermatology and sexually transmitted diseases. Manuscripts may be submitted in the form of editorials, original articles, review articles, case reports, therapeutical notes, special articles and letters to the Editor. Manuscripts are expected to comply with the instructions to authors which conform to the Uniform Requirements for Manuscripts Submitted to Biomedical Editors by the International Committee of Medical Journal Editors (www.icmje.org). Articles not conforming to international standards will not be considered for acceptance.