嘌呤核苷酶解法合成细胞分裂素。

Q4 Chemistry

Current Protocols in Nucleic Acid Chemistry Pub Date : 2018-12-01 Epub Date: 2018-10-09 DOI:10.1002/cpnc.61

Vladimir E Oslovsky, Mikhail S Drenichev, Cyril S Alexeev, Pavel N Solyev, Roman S Esipov, Sergey N Mikhailov

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引用次数: 3

摘要

本单元描述了一种制备天然细胞分裂素及其合成衍生物的有效方法，该方法是在嘌呤核苷磷酸化酶(PNP)和Na2 HAsO4存在下，酶切N6 -取代腺苷或O6 -取代肌苷衍生物的n -糖苷键。由于α- d -核糖-1-砷酸盐被水解，砷解反应是不可逆的。结果，通过高效液相色谱(HPLC)分析表明，所需产品的产量接近定量，并且易于分离。在这里使用的策略中，核糖残基充当保护基团。©2018 by John Wiley & Sons, Inc。

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Synthesis of Cytokinins via Enzymatic Arsenolysis of Purine Nucleosides.

This unit describes an effective method for the preparation of natural cytokinins and their synthetic derivatives based on enzymatic cleavage of the N-glycosidic bond of N⁶ -substituted adenosine or O⁶ -substituted inosine derivatives in the presence of purine nucleoside phosphorylase (PNP) and Na₂ HAsO₄ . The arsenolysis reaction is irreversible due to the hydrolysis of the resulting α-D-ribose-1-arsenate. As a result, the desired products are formed in near-quantitative yields, as indicated by high-performance liquid chromatography (HPLC) analysis, and can easily be isolated. In the strategy used here, the ribose residue acts as a protective group. © 2018 by John Wiley & Sons, Inc.

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来源期刊

Current Protocols in Nucleic Acid Chemistry Chemistry-Organic Chemistry

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期刊介绍： Published in association with International Society for Nucleosides, Nucleotides & Nucleic Acids (IS3NA) , Current Protocols in Nucleic Acid Chemistry is equally valuable for biotech, pharmaceutical, and academic labs. It is the resource for designing and running successful research projects in the rapidly growing and changing field of nucleic acid, nucleotide, and nucleoside research.