{"title":"3-氨基苯甲酰胺对暴露于甲磺酸甲酯的哺乳动物细胞中二价阳离子依赖性DNA片段的增强作用。","authors":"J K Park, I S Park, J S Lee, S D Park","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>THE PROPERTIES OF METHYL METHANESULFONATE (MMS) DAMAGE-RELATED NUCLEASE ACTIVITY IN CHO AND HELA CELLS WERE STUDIED BY NEUTRAL AND ALKALINE ELUTION TECHNIQUES. THE FRAGMENTATION OF MMS-TREATED DNA WAS DEPENDENT ON THE PRESENCE OF CA++ AND MG++ IONS, AND WAS NOT OBSERVED UPON TREATMENT WITH DIVALENT ION CHELATORS SUCH AS EDTA OR EGTA. THE CA++ AND MG++ -DEPENDENT CONVERSION OF MMS-TREATED SUPERCOILED PLASMID PREVIOUSLY REACTED WITH CHO CELL NUCLEAR EXTRACT TO OPEN CIRCULAR FORM WAS MARKEDLY INCREASED IN A 3-AMINOBENZAMIDE (3-AB) DOSE-DEPENDENT MANNER AS DETERMINED BY AGAROSE GEL ELECTROPHORESIS AND DENSITOMETRIC ANALYSIS. THESE RESULTS SUGGEST THAT THE INHIBITION OF POLY (ADP-RIBOSE) POLYMERASE BY 3-AB RESULTS IN THE ACTIVATION OF THE CA++, MG++ -DEPENDENT ENDONUCLEASE WHICH NONSPECIFICALLY CUTS MMS-TREATED DNA.</p>","PeriodicalId":89152,"journal":{"name":"In vitro toxicology","volume":"4 1","pages":"73-9"},"PeriodicalIF":0.0000,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"POTENTIATION EFFECTS OF 3-AMINOBENZAMIDE ON DIVALENT CATION-DEPENDENT DNA FRAGMENTATION IN MAMMALIAN CELLS EXPOSED TO METHYL METHANESULFONATE.\",\"authors\":\"J K Park, I S Park, J S Lee, S D Park\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>THE PROPERTIES OF METHYL METHANESULFONATE (MMS) DAMAGE-RELATED NUCLEASE ACTIVITY IN CHO AND HELA CELLS WERE STUDIED BY NEUTRAL AND ALKALINE ELUTION TECHNIQUES. THE FRAGMENTATION OF MMS-TREATED DNA WAS DEPENDENT ON THE PRESENCE OF CA++ AND MG++ IONS, AND WAS NOT OBSERVED UPON TREATMENT WITH DIVALENT ION CHELATORS SUCH AS EDTA OR EGTA. THE CA++ AND MG++ -DEPENDENT CONVERSION OF MMS-TREATED SUPERCOILED PLASMID PREVIOUSLY REACTED WITH CHO CELL NUCLEAR EXTRACT TO OPEN CIRCULAR FORM WAS MARKEDLY INCREASED IN A 3-AMINOBENZAMIDE (3-AB) DOSE-DEPENDENT MANNER AS DETERMINED BY AGAROSE GEL ELECTROPHORESIS AND DENSITOMETRIC ANALYSIS. THESE RESULTS SUGGEST THAT THE INHIBITION OF POLY (ADP-RIBOSE) POLYMERASE BY 3-AB RESULTS IN THE ACTIVATION OF THE CA++, MG++ -DEPENDENT ENDONUCLEASE WHICH NONSPECIFICALLY CUTS MMS-TREATED DNA.</p>\",\"PeriodicalId\":89152,\"journal\":{\"name\":\"In vitro toxicology\",\"volume\":\"4 1\",\"pages\":\"73-9\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1991-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"In vitro toxicology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"In vitro toxicology","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
POTENTIATION EFFECTS OF 3-AMINOBENZAMIDE ON DIVALENT CATION-DEPENDENT DNA FRAGMENTATION IN MAMMALIAN CELLS EXPOSED TO METHYL METHANESULFONATE.
THE PROPERTIES OF METHYL METHANESULFONATE (MMS) DAMAGE-RELATED NUCLEASE ACTIVITY IN CHO AND HELA CELLS WERE STUDIED BY NEUTRAL AND ALKALINE ELUTION TECHNIQUES. THE FRAGMENTATION OF MMS-TREATED DNA WAS DEPENDENT ON THE PRESENCE OF CA++ AND MG++ IONS, AND WAS NOT OBSERVED UPON TREATMENT WITH DIVALENT ION CHELATORS SUCH AS EDTA OR EGTA. THE CA++ AND MG++ -DEPENDENT CONVERSION OF MMS-TREATED SUPERCOILED PLASMID PREVIOUSLY REACTED WITH CHO CELL NUCLEAR EXTRACT TO OPEN CIRCULAR FORM WAS MARKEDLY INCREASED IN A 3-AMINOBENZAMIDE (3-AB) DOSE-DEPENDENT MANNER AS DETERMINED BY AGAROSE GEL ELECTROPHORESIS AND DENSITOMETRIC ANALYSIS. THESE RESULTS SUGGEST THAT THE INHIBITION OF POLY (ADP-RIBOSE) POLYMERASE BY 3-AB RESULTS IN THE ACTIVATION OF THE CA++, MG++ -DEPENDENT ENDONUCLEASE WHICH NONSPECIFICALLY CUTS MMS-TREATED DNA.
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