Camila Cohen Kaleka , Eder Zucconi , Tierri da Silva Vieira , Mariane Secco , Mário Ferretti , Moisés Cohen
{"title":"不同商用透明质酸作为人脂肪源间充质干细胞注射载体的评价","authors":"Camila Cohen Kaleka , Eder Zucconi , Tierri da Silva Vieira , Mariane Secco , Mário Ferretti , Moisés Cohen","doi":"10.1016/j.rboe.2018.07.009","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><p>The main purpose of this study is to evaluate, <em>in vitro</em>, the cytotoxicity of different commercial brands of hyaluronic acids to be used as a vehicle for injection of human adipose-derived mesenchymal stem cells (AD-MSCs).</p></div><div><h3>Methods</h3><p>AD-MSCs were divided into seven groups: one control group where AD-MSCs were cultivated with phosphate-buffered saline (PBS) and six other groups where AD-MSCs were cultivated with different commercial brands of hyaluronic acid. AD-MSC viability analysis was performed after 4, 24, and 48<!--> <!-->h in contact with each treatment, using the trypan staining method on a Countess automated cell counter (Thermo Fisher Scientific).</p></div><div><h3>Results</h3><p>The results clearly demonstrated a significant difference in cell viability when AD-MSCs were exposed to different hyaluronic acids when compared with the control group.</p></div><div><h3>Conclusion</h3><p>These data suggest that hyaluronic acid can be used as a vehicle for injection of human AD-MSCs, but caution is needed to choose the best product, aiming at its future therapeutic application.</p></div>","PeriodicalId":101095,"journal":{"name":"Revista Brasileira de Ortopedia (English Edition)","volume":"53 5","pages":"Pages 557-563"},"PeriodicalIF":0.0000,"publicationDate":"2018-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.rboe.2018.07.009","citationCount":"6","resultStr":"{\"title\":\"Evaluation of different commercial hyaluronic acids as a vehicle for injection of human adipose-derived mesenchymal stem cells\",\"authors\":\"Camila Cohen Kaleka , Eder Zucconi , Tierri da Silva Vieira , Mariane Secco , Mário Ferretti , Moisés Cohen\",\"doi\":\"10.1016/j.rboe.2018.07.009\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objective</h3><p>The main purpose of this study is to evaluate, <em>in vitro</em>, the cytotoxicity of different commercial brands of hyaluronic acids to be used as a vehicle for injection of human adipose-derived mesenchymal stem cells (AD-MSCs).</p></div><div><h3>Methods</h3><p>AD-MSCs were divided into seven groups: one control group where AD-MSCs were cultivated with phosphate-buffered saline (PBS) and six other groups where AD-MSCs were cultivated with different commercial brands of hyaluronic acid. AD-MSC viability analysis was performed after 4, 24, and 48<!--> <!-->h in contact with each treatment, using the trypan staining method on a Countess automated cell counter (Thermo Fisher Scientific).</p></div><div><h3>Results</h3><p>The results clearly demonstrated a significant difference in cell viability when AD-MSCs were exposed to different hyaluronic acids when compared with the control group.</p></div><div><h3>Conclusion</h3><p>These data suggest that hyaluronic acid can be used as a vehicle for injection of human AD-MSCs, but caution is needed to choose the best product, aiming at its future therapeutic application.</p></div>\",\"PeriodicalId\":101095,\"journal\":{\"name\":\"Revista Brasileira de Ortopedia (English Edition)\",\"volume\":\"53 5\",\"pages\":\"Pages 557-563\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2018-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.rboe.2018.07.009\",\"citationCount\":\"6\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Revista Brasileira de Ortopedia (English Edition)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2255497118300983\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Revista Brasileira de Ortopedia (English Edition)","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2255497118300983","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Evaluation of different commercial hyaluronic acids as a vehicle for injection of human adipose-derived mesenchymal stem cells
Objective
The main purpose of this study is to evaluate, in vitro, the cytotoxicity of different commercial brands of hyaluronic acids to be used as a vehicle for injection of human adipose-derived mesenchymal stem cells (AD-MSCs).
Methods
AD-MSCs were divided into seven groups: one control group where AD-MSCs were cultivated with phosphate-buffered saline (PBS) and six other groups where AD-MSCs were cultivated with different commercial brands of hyaluronic acid. AD-MSC viability analysis was performed after 4, 24, and 48 h in contact with each treatment, using the trypan staining method on a Countess automated cell counter (Thermo Fisher Scientific).
Results
The results clearly demonstrated a significant difference in cell viability when AD-MSCs were exposed to different hyaluronic acids when compared with the control group.
Conclusion
These data suggest that hyaluronic acid can be used as a vehicle for injection of human AD-MSCs, but caution is needed to choose the best product, aiming at its future therapeutic application.
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