[肺复康米斯图拉化学成分及含量测定研究]。

中药材 Pub Date : 2016-08-01

Yan Ren, Xiang-ming Chen, Juan-juan Zhao, Chang-jun Lv

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引用次数: 0

摘要

目的:鉴别肺复康中主要化学成分并测定其含量。方法:采用高效液相色谱-质谱技术，通过保留时间、质谱数据与参比标准品的对比，对其化学成分进行定性分析。采用高效液相色谱法测定各化学成分的含量。结果:从肺复康中分离得到5个化合物，鉴定为新桂皮素、芒果苷、毛蕊花苷-7- o -葡萄糖苷、毛蕊花苷、五味子甲素a，其标准曲线在2范围内呈良好的线性关系。08 ~ 104。0 μg/m L,2。00 ~ 100。0 μg/m L,2。00 ~ 100。0μg / m L, 2。09 ~ 104。5 μg/m L;98 ~ 99。分别为0 μg/m L。平均回收率均在91的范围内。3 ~ 103。8%。结论:建立了肺复康药材化学成分鉴定及含量测定方法，可更好地揭示肺复康药材的物质基础和质量控制。

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[Study on Chemical Constituents and Content Determination of Feifukang Mistura].

Objective: To identify the main chemical constituents and to determine the content in Feifukang mistura.

Methods: HPLC-MS technique was used to profile and identify the chemical constituents by comparing the retention time,MS data with the reference standard. The content determination of all the chemical constituents were carried on a HPLC system.

Results: Five compounds were separated from Feifukang mistura,which were identified as neomangiferin,mangiferin,calycosin-7-O-glucoside,calycosin,and schizandrol A. The standard curves of them showed good linearity on the range of 2. 08 ~ 104. 0 μg/m L,2. 00 ~ 100. 0 μg/m L,2. 00 ~ 100. 0μg/m L,2. 09 ~ 104. 5 μg/m L,and 1. 98 ~ 99. 0 μg/m L,respectively. The average recoveries were all in the range of 91. 3 ~ 103. 8%.

Conclusion: The methods of chemical constituents identification and content determination were established,which may offer better revealing the material basis and controlling quality of Feifukang mistura.

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来源期刊

中药材

自引率

0.00%

发文量

11364

期刊介绍： "Chinese Materia Medica" magazine was founded in 1978. It is a national-level academic journal of Chinese medicine science and technology approved by the State Science and Technology Commission and the Press and Publication Administration for public distribution at home and abroad. It is sponsored by the Chinese Materia Medica Information Center of the State Food and Drug Administration and is publicly distributed at home and abroad. It mainly reports on new technologies, new methods and new achievements in the production, scientific research and business management of Chinese medicine, and serves to promote the production and scientific and technological development of Chinese medicine, improve the output and quality of Chinese medicine, improve business management, and prosper the Chinese medicine industry.